形态学与形态计量学观察大豆异黄酮对前列腺增生大鼠的作用  

Morphological and Morphometric Study on the Effect of Soybean Isoflavone on Hyperplastic Prostate in Rats

作　　者：任国峰[1] 袁泉[1] 杨爱青[1] 汤凌[1] 黄忆明[1] 

机构地区：[1]中南大学公共卫生学院营养与食品卫生学系,长沙410078

出　　处：《中国实验动物学报》2010年第1期24-27,I0006,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助(编号:30500407)

摘　　要：目的探讨不同剂量大豆异黄酮(SI)对前列腺增生大鼠组织形态学和超微结构的影响。方法应用丙酸睾酮诱导雄性SD大鼠前列腺增生,随机分为五组:正常对照组、模型组和3个大豆异黄酮剂量组,分别灌胃SI 60、120、240 mg/(kg.d),28 d后,光镜观察前列腺组织形态,同时结合图像分析系统检测前列腺腺体、间质的形态计量学改变,透射电镜观察前列腺细胞超微结构的变化。结果各剂量大豆异黄酮均可降低前列腺增生大鼠前列腺湿重、指数及体积,降低上皮细胞高度、腺体面积、腺体相对总体积、单位体积内腺体平均直径、平均体积、平均表面积和间质相对总体积,提高腺体数目、腺体数密度、腺体表面积/腺体体积和腺体平均曲率。结论大豆异黄酮具有抑制大鼠前列腺增生的作用。Objective To investigate the effect of soybean isoflavone on histology and ultrastructure of benign prostatic hyperplasia in rats.Methods Male SD rats were injected subcutaneously testosterone propionate for 28 d to induce prostatic hyperplasia,and the rats were divided into 5 groups： control group,model group,and 3 test groups with SI in a dose of 60 mg/（kg·d）,120 mg/（kg·d） and 240 mg/（kg·d）,respectively.The wet prostate weight,prostatic index,morphological,ultrastructural and morphometric changes of the prostatic glandular and interstitial tissues were observed.Results The prostate wet weight,prostatic index and prostatic volumes in all dose groups were significantly lower than those in the models.In comparison with the model group,height of prostatic epithelial cells,glandular average diameters,volumes and surface areas in unit volume,as well as glandular circumferences,glandular relative total volumes and interstitial relative total volumes were all significantly decreased.Glandular counts,density,ratio of glandular surface area to volume,and glandular average curvature were all increased.Conclusions Soybean isoflavone can inhibit prostatic hyperplasia in rats.

关 键 词：大豆异黄酮 前列腺增生 形态计量学 超微结构 

分 类 号：R151.1[医药卫生—营养与食品卫生学]