牙源性角化囊性瘤骨吸收相关因子组织化学及免疫组织化学双重染色表达  

Histochemical and immunohistochemical double staining for osteolytic related factors in keratocystic odontogenic tumor

作　　者：王恩博[1] 崔念晖[1] 柴原孝彦[2] 野村武史[2] 张伟[1] 俞光岩[1] 

机构地区：[1]北京大学口腔医学院.口腔医院口腔颌面外科,北京100081 [2]日本东京齿科大学口腔外科

出　　处：《北京大学学报（医学版）》2010年第1期85-89,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金项目(30600711)资助~~

摘　　要：目的:应用组织化学及免疫组化双重染色法研究几种骨吸收相关因子如细胞核因子,κβ受体活化因子配体(receptor activator of nuclear factor-κB ligand,RANKL)、骨保护素(osteoprotegerin,OPG)、白细胞介素1α(inter-leukin 1α,IL-1α)、甲状旁腺激素相关蛋白(parathyroid hormone-related protein,PTHrP)和破骨细胞标志物抗酒石酸磷酸酶(tartrate-resistant acid phosphatase staining,TRAP)在牙源性角化囊性瘤(KCOT)囊壁组织上的表达特点。方法:20例KCOT石蜡标本连续切片5张,1张HE染色核实诊断,其余4张采用组织化学和免疫组织化学双重染色法,分别检测TRAP、RANKL、OPG、IL-1α和PTHrP抗体在KCOT囊壁组织上的表达。结果:KCOT囊壁组织上分别有TRAP、RANKL、OPG、IL-1α和PTHrP的阳性表达,其中TRAP和RANKL主要表达在囊壁结缔组织与骨交界面位置。10例有TRAP阳性表达,12例RANKL阳性表达可位于纤维结缔组织内、血管腔周围和上皮层。TRAP和RANKL双阳性表达5例。OPG在囊肿上阳性表达少。IL-1α在上皮层和纤维结缔组织层均有表达,但阳性细胞主要是棘细胞层和表层细胞。PTHrP也可在上皮细胞层和结缔组织层表达。结论:RANKL、IL-1α和PTHrP等骨吸收相关因子参与调节牙源性角化囊性瘤中破骨细胞的分化和活化,导致骨吸收。Objective：To investigate the activities of bone resorption related cytokines at the capsule wall of the keratocystic odontogenic tumor（KCOT） by histochemical and immunohistochemical double staining for tartrate-resistant acid phosphatase staining（TRAP）,receptor activator of nuclear factor-κB ligand（RANKL）,osteoprotegerin（OPG）,interleukin1α（IL-1α）and parathyroid hormone-related protein（PTHrP）.Methods： Twenty paraffin blocks of KCOT were sectioned in sequence into five slices.One slice was stained with hematoxylin and eosin to confirm pathological diagnosis.Each of other four slices was histochemically and immunohistochemically double stained separately by TRAP with RANKL,TRAP with OPG.TRAP with IL-1α and TRAP with PTHrP to observe expressions of these factors in KCOT.Results： TRAP positive expression was shown in 10 cases.Positive expression of both TRAP and RANKL were detected in 5 cases.RANKL positive staining mainly scattered in fibrous connective tissue,blood vessels and epithelium was shown in 12 cases.OPG positive staining was detected in 4 cases.IL-1α positive cells were also observed in 10 cases and PTHrP positive cells were seen in 6 cases.The TRAP and RANKL positive cells were scattered within the connective tissues near the bone.The IL-1α positive staining cells were observed both in epithelium and connective tissues,but predominately in the former.Conclusion： The mechanism of osteoclastogenesis of KCOT may also be mediated by osteolytic factors such as RANKL,IL-1α and PTHrP.These factors involved in the activation and differentiation of pre-osteoclasts to form osteoclasts,thus finally resulted in jaw bone resorption.

关 键 词：牙源性囊肿 破骨细胞 细胞因子类 免疫组织化学 

分 类 号：R782[医药卫生—口腔医学]