虎奶菇菌丝体自溶条件的优化研究  

Study on the Optimization of the Autolytic Conditions of Pleurotus tuber-regium (Fr.) Sing Mycelium

作　　者：黄碧芳[1] 陈婷[1] 黄杰[1] 邱莉莉[1] 林文雄[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002

出　　处：《中国农学通报》2010年第5期254-256,共3页Chinese Agricultural Science Bulletin

基　　金：福建农林大学校青年基金项目"虎奶菇菌丝自溶的差异蛋白组学分析"(06A10)

摘　　要：自溶现象广泛存在于各种微生物中，但有关食药用菌虎奶菇（Pleurotus tuber-regium （Fr.） Sing.）菌丝体自溶的研究国内外均未见报道。本文利用液体发酵的方法来培养虎奶菇菌丝体，并分别研究了不同温度、pH值与渗透压、O2供应等因素对其菌丝体自溶的影响。结果表明，在本实验条件下，虎奶菇菌丝体较佳的单因子自溶条件分别为温度40℃、pH 9.0、NaCl浓度3-4%及无氧条件。本实验结果为虎奶菇菌丝体自溶机制的深入研究奠定了基础。The phenomenon of autolysis is widespread among various kinds of microbes. However, there is hitherto no report on the autolysis of Pleurotus tuber-regium （Ft.） Sing. Liquid fermentation method was applied to culture the Pleurotus tuber-regium （Ft.） Sing mycelia and the effects of temperature, pH, osmotic pressure and oxygen supply on the autolysis of Pleurotus tuber-regium （Ft.） Sing mycelium were examined. The results indicated that the optimal conditions for the mycelium autolysis were 40℃, pH 9.0, 3%-4%NACl and anaerobic fermentation. This study provides the basis on which the further investigation of the Pleurotus tuber-regium （Ft.） Sing mycelium autolysis mechanism can be conducted.

关 键 词：虎奶菇 自溶条件 优化 

分 类 号：S3[农业科学—农艺学]