鸭A-FABP基因内含子1多态性与肌内脂肪和血清甘油三酯含量相关研究  

Association of the Polymorphism in Intron-1 of A-FABP Gene with the Content of Intramuscular Fat and Serum Triglyceride in Ducks

作　　者：张海波[1] 徐琪[1] 张依裕[1,2] 段修军 孙莉[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]贵州大学动物科学学院,贵阳550025 [3]江苏省畜牧兽医职业技术学院,泰州225300

出　　处：《畜牧兽医学报》2010年第2期155-161,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技部支撑计划重大专项(2006BDA01A09);国家科技部支撑计划重点项目(2006BDA14B06)

摘　　要：本研究旨在探讨鸭脂肪型脂肪酸结合蛋白(Adipocyte Fatty Acid-Binding Protein,A-FABP)基因部分序列多态与鸭肌内脂肪和血清甘油三酯含量关系。根据鸭A-FABPmRNA序列和鸡A-FABP基因组序列设计1对引物扩增鸭A-FABP基因内含子1序列。同时根据扩增产物设计4对引物,利用PCR-SSCP对鸭A-FABP基因部分序列进行多态性研究并探究序列多态性与鸭肌内脂肪和血清甘油三酯含量的关系。结果表明:克隆序列包含鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与鸡A-FABP基因内含子1同源性为75.1%;经SS-CP检测,发现引物P1扩增片段有3处碱基突变,引物P3有6处碱基突变,这些突变分别产生了3种和8种基因型。在P1位点樱桃谷鸭和苏牧麻鸭偏离了Hardy-Weinberg平衡(P<0.01),金定鸭基因型分布符合Hardy-Weinberg平衡(P>0.05),在P3位点3个群体均符合Hardy-Weinberg平衡(P>0.05)。同时,这些基因型在不同群体中分布存在显著或极显著差异(P<0.05或P<0.01)。最小二乘分析显示,肌内脂肪和血清甘油三酯含量在各群体中有显著差异(P<0.05),P1和P3位点各基因型对肌内脂肪含量无显著影响(P>0.05),仅在P3位点CD基因型个体血清甘油三酯含量显著高于其他基因型个体(P<0.05)。结果提示,鸭A-FABP基因内含子1多态对血清甘油三酯含量有一定影响,但是否影响鸭肌内脂肪沉积,还需进一步研究。This experiment was conducted to study the association of the polymorphism of partial Adipocyte Fatty Acid-Binding Protein（A-FABP） gene with the content of intramuscular fat and serum Triglyceride in ducks.A pair of primers P-in1 was designed according to the sequences of A-FABP gene mRNA in duck and A-FABP gene in chicken to amplify duck intron-1 of A-FABP gene,then 4 pairs of primers were designed according to the cloned gene.The SNPs were detected by PCR-SSCP,confirmed by sequencing,then the association of polymorphism of A-FABP gene with the content of intramuscular fat and serum Triglyceride was studied.The results indicated that the product by P-in1 included partial exon-1,exon-2 and complete intron-1 of A-FABP gene after clone and sequence,and homologous comparison was done between duck and chicken A-FABP gene,which showed the homology was 75.1%.Three and six nucleotide mutations were found in the products by primer P1 and P3,respectively,which resulted in three and eight genotypes,respectively.The results of Chi-Square test showed that Cherry Valley duck and Sumu duck deviated from the Hardy-Weinberg equilibrium（P〈0.01） except Jingding duck in P1 locus,and all of the three groups fit with it in P3 locus（P〉0.05）,while the distribution of them was different in various groups（P〈0.05 or P〉0.01） at the same time.The least square analysis showed that the content of IMF and TG were significant difference in different groups（P〈0.05）,but the content of IMF had no significant difference between genotypes in P1 and P3（P〉0.05）,only content of TG had significant difference in various genotypes in P3 and the ducks with CD genotype had higher content of TG than those with other genotypes（P〈0.05）.These results suggested that polymorphism in intron-1 of A-FABP gene have certain effect on TG content,also indicated that it needs further study to get a conclusion if these SNPs can influence the intramuscular fat trait in duck.

关 键 词：鸭 A-FABP基因 克隆 SSCP 肌内脂肪 血清甘油三酯 

分 类 号：S831.2[农业科学—畜牧学]