禁食对生长期北京鸭腺胃ghrelin免疫阳性细胞的影响  

Effects of Fasting on the Ghrelin Immunopositive Cells in the Proventriculus in Growing Peking Ducks

作　　者：邵育静[1] 张书宁[1] 黄鹤悠[1] 孙鹏[1] 唐小艳[1] 韩海棠[1] 李赞东[1] 

机构地区：[1]中国农业大学生物学院农业生物技术国家重点实验室,北京100193

出　　处：《畜牧兽医学报》2010年第2期224-228,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高新技术研究发展计划"863"计划(2007AA100504)

摘　　要：本研究旨在观察禁食是否对生长期北京鸭腺胃ghrelin免疫阳性细胞生成有影响。用免疫组织化学的方法检测25、35、50日龄的北京鸭(已经禁食72 h)单位面积(mm2)腺胃组织中ghrelin阳性细胞数目。研究发现在这3个时期,不管是禁食组还是自由采食组,大量ghrelin阳性细胞多分布于腺胃深层复管状腺中;禁食组腺胃中gh-relin阳性细胞数目比自由采食组极显著增加(P<0.01)。结果提示:禁食是影响腺胃产生有活性ghrelin的重要因素。This experiment was conducted to examine whether fasting affect ghrelin-immunopositive（ghrelin-ip） cells in the proventriculus in Peking ducks.This is a test-report of ghrelin-ip cells/mm^2 in proventriculus from 25,35,50-day-old in Peking duck ad libitum and fasted for 72 hours by immunohistochemistry analysis.Datas showed that many strongly ghrelin-ip cells were observed in the compound tublar glands in the proventriculus whether in the fasting group or in the free feed group in above three periods.The numbers of ghrelin-ip cells/mm^2 were significantly increased compared with the free feed group（P〈0.01）.The results demonstrate that feeding status influence the immunopositive ghrelin levels.

关 键 词：北京鸭 GHRELIN 禁食 免疫组织化学 腺胃 

分 类 号：S852.2[农业科学—基础兽医学]