角果木dolabrane型二萜对Lewis荷瘤小鼠的抗肿瘤作用  

Anti-tumor effect of the dolabrane diterpenes of ceriops tagal in Lewis lung carcinoma mice

作　　者：孙蓓[1] 王秀美[1] 邱文生[1] 张红军[1] 梁军[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003

出　　处：《临床肿瘤学杂志》2010年第2期127-131,共5页Chinese Clinical Oncology

摘　　要：目的研究角果木dolabrane型二萜对Lewis荷瘤小鼠皮下移植瘤的抑制作用,并初步探讨其作用机制。方法建立Lewis荷瘤小鼠皮下移植瘤模型,随机分为角果木dolabrane型二萜低、中、高剂量组(5、10、20mg/kg)、生理盐水组及环磷酰胺组(CTX 10mg/kg),各组连续给药10天,停药后24小时处死小鼠,称取各组瘤重,计算抑瘤率,观察抑瘤作用。应用流式细胞技术测定肿瘤细胞凋亡率,免疫组化方法检测肿瘤相关转录因子NF-κBp65的表达水平。结果角果木dolabrane型二萜对肿瘤生长有明显的抑制作用,高、中、低剂量组抑瘤率分别为44.12%、25.74%和22.59%,与生理盐水空白对照组比较差异有统计学意义(P<0.05),而与CTX阳性对照组比较差异无统计学意义(P>0.05)。角果木dolabrane型二萜各剂量组均未出现明显的脏器毒性。肿瘤细胞的凋亡率随着角果木dolabrane型二萜剂量的增加而增高,而NF-κBp65的表达水平则随其剂量的增加而降低,差异具有统计学意义(P<0.05)。结论角果木dolabrane型二萜对Lewis荷瘤小鼠皮下移植瘤的生长具有较明显的抑制作用,且其作用机制可能与肿瘤凋亡和相关转录因子NF-κBp65的表达水平降低有关。Objective To preliminarily study the anti-tumor effect of dolabrane diterpenes of ceriops tagal in Lewis lung carcinoma mice and its mechanism. Methods After the establishment of subcutaneously transplanted solid tumor models with Lewis lung carcinoma cell, all the models were randomly divided into 5 groups ： 5, 10 and 20 mg/kg dolabrane diterpenes groups, saline group as tumor control group and CTX （10 mg/kg） group as positive group, respectively. Then 10 days successive administration were given to all the carcinoma models and the mice were killed 24 hours after the last medication. Then the tumors of each group were weighted and the tumor inhibition rates were calculated, as well as detecting the apoptosis rates using flow-cytometry and the expression level of the tumor-associated transcription factor NF-KBp65 using immunohistoehemistry respectively. Results The tumor inhibition rates of the 20, 10 and 5mg/kg dolabrane diterpenes groups were 44. 12% , 25.74% and 22. 59%. Compared to the tumor control group, there were obvious tumor inhibition effects in dolabrane diterpenes groups and each group did not have obvious organ toxicity （ P 〈0. 05 ）. The apoptosis rates positively correlated with the dosage of dolabrane diterpenes. And the expression levels of the NF-κBp65 in the experimental groups were lower than that in the control group （ P 〈 0. 05 ）. Conclusion The dolabrane diterpenes of ceriops tagal has significant anti-tumor effect in the Lewis lung carcinoma mice and the mechanism may associate with the induced apoptosis of tumor cell and the lower expression levels of the tumor-associated transcription factor NF-κBp65.

关 键 词：角果木dolabrane型二萜 Lewis荷瘤小鼠 抑瘤作用 凋亡 NF—κBp65 

分 类 号：R734.2[医药卫生—肿瘤]