重楼复方与氟尿嘧啶对胃癌细胞的体外协同作用研究  

In vitro synergistic interaction between CLFF and 5-fluorouracil on human gastric cancer cell lines

作　　者：刘永萍[1,2,3] 凌扬[2] 胡文静[3] 谢丽[3] 禹立霞[3] 钱晓萍[3] 刘宝瑞[3] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]苏州大学附属常州市肿瘤医院临床肿瘤学实验室,江苏常州213002 [3]南京中医药大学南京鼓楼医院肿瘤中心,江苏南京210008

出　　处：《现代肿瘤医学》2010年第3期442-445,共4页Journal of Modern Oncology

基　　金：江苏省常州市科技计划项目(编号:CS20092025)

摘　　要：目的:探讨重楼复方(Chong Lou Fu Fang,CLFF)与5-氟尿嘧啶(5-fluorouracil,5-FU)对胃癌细胞有无体外协同作用及CLFF对胸苷酸合成酶(thymidylate synthase,TS)基因表达和细胞凋亡的影响。方法:MTT法检测细胞增殖能力;基于中位效应原则的联合指数法评估CLFF与5-FU对胃癌细胞系SGC-7901和BGC-823的相互作用;Annexin-Ⅴ-FITC和PI双染色法检测细胞凋亡率;荧光定量RT-PCR法测定TS和β-actinmRNA表达。结果:CLFF在两株细胞中与5-FU均产生较好的协同细胞毒性,两药合用亦以协同方式诱导肿瘤细胞凋亡;CLFF作用24h后,SGC-7901和BGC-823细胞中TSmRNA表达水平分别为对照组的(0.30±0.03)倍和(0.46±0.03)倍,用药前后两株细胞中TSmRNA表达水平具有统计学差异。结论:CLFF与5-FU有较好的协同抗肿瘤作用,可能与二者协同诱导肿瘤细胞凋亡以及CLFF可下调胸苷酸合成酶基因表达有关。Objective：To assess the synergistic interaction between CLFF （Chong Lou Fu Fang） and 5 -fluorou racil （5 -FU） in gastric cancer cells, and to investigate the influence of CLFF on thymidylate synthase gene expres sion and cell apoptosis. Methods：The cell proliferation capacity was determined by MTF assay. Interaction betweel CLFF and 5 - FU in human gastric cancer SGC - 7901 and BGC - 823 cells was evaluated using the combination in dex （CI） method based on median - effect principle. Apoptosis rates of cancer cells were evaluated by means of flo~ cytometry. Expression of thymidylate synthase gene with or without CLFF treatment were measured by real time quanti tative PCR. Results： CLFF had a synergistic effect on the cytotoxicity of 5 - FU in both two gastric cancer cell lines. The combination of CLFF and 5 - FU could also induce apoptosis in a synergistic manner. TS mRNA levels in botl cells were markedly suppressed at （0.30± 0.03 ） and （0.46 ± 0.03 ） fold, respectively, by the presentation of CLFF for 24h. Compared with the levels before CLFF treatment, the difference was significant. Concluslon：The combinatiol of CLFF and 5 - FU showed synergistic antitumor activity in both two gastric cancer cell lines. The possible mecha. nisms might be the synergistic apoptotic effect and the downregulation of thymidylate synthase gene.

关 键 词：氟尿嘧啶 胸苷酸合成酶 凋亡 胃癌细胞系 

分 类 号：R73-361[医药卫生—肿瘤] R735.2[医药卫生—临床医学]