DC-CIK细胞抗鼻咽癌细胞的作用  

The anti-tumor effect of DC-CIK cells against nasopharyngeal carcinoma cells

作　　者：方慧云[1] 程伟民[1] 李晓玲[1] 季明芳[1] 

机构地区：[1]中山市人民医院肿瘤研究所,广东中山528403

出　　处：《现代肿瘤医学》2010年第3期469-470,共2页Journal of Modern Oncology

摘　　要：目的:研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后DC-CIK细胞抗鼻咽癌细胞的作用。方法:正常人外周血单个核细胞诱导DC和CIK细胞,加入CNE1或CNE2细胞冻融液,将DC与CIK共培养,以CIK细胞单独培养为对照。用MTT法测定杀伤活性。结果:DC-CIK细胞杀伤鼻咽癌细胞活性高于CIK细胞(P<0.05);杀伤CNE1细胞活性高于CNE2细胞(P<0.05)。结论:DC-CIK细胞抗鼻咽癌细胞的作用显著,为DC-CIK细胞免疫治疗提供了实验和理论依据。Objective：To investigate the anti -tumor activity against nasopharyngeal carcinoma cells of DC - CIK cells in co - culture of cytokine induced killer （CIK） cells with dendritic cells （DC）. Methods ： DC and CIK were prepared from healthy human peripheral blood mononuclear cells. Mixing CNE1 cells or CNE2 cells, they were co - cultured peripherally. CIK cells were cultured alone as controls, killing activity was detected by MTT assay. Results： The killing activity of DC - CIK cells was significantly higher than that of CIK cells （ P 〈 0.05 ）. The anti - tumor activity against CNE1 cells was significantly higher than that of CNE2 cells （ P 〈 0.05 ）. Conclusion：The anti - tumor effect against nasopharyngeal carcinoma cells of DC - CIK cells were significantly high.

关 键 词：CIK细胞 DC-CIK细胞 鼻咽癌 

分 类 号：R730.51[医药卫生—肿瘤] R739.63[医药卫生—临床医学]