高强度聚焦超声对转移性肝癌患者机体免疫功能的影响  

Changes in the immune function of patients with metastatic hepatic carcinoma after high intensity focused ultrasound treatment

作　　者：吴发伟[1] 冯蕾[2] 党亚正[1] 曹玮[3] 齐涛[1] 蔡永胜[1] 

机构地区：[1]解放军第323医院肿瘤中心,陕西西安710054 [2]第四军医大学遗传与发育教研室,陕西西安710032 [3]第四军医大学唐都医院介入科,陕西西安710038

出　　处：《现代肿瘤医学》2010年第3期517-519,共3页Journal of Modern Oncology

摘　　要：目的:观察高强度聚焦超声(high intensity focused ultrasound,HIFU)对转移性肝癌患者机体免疫功能的影响。方法:转移性肝癌患者30例,通过全身麻醉、超声肿瘤定位进行HIFU治疗,采用流式细胞仪及双抗体夹心法,检测治疗前、后外周血T细胞亚群(CD3+、CD4+、CD8+)、NK细胞的百分率及sIL-2R的变化。采用LDH释放法检测NK细胞的杀伤活性。结果:HIFU治疗后CD3+、CD4+、NK细胞的百分率明显升高(P<0.05),CD8+细胞的百分率明显降低(P<0.05),NK细胞杀伤活性较治疗前升高,但无统计学差异(P>0.05);sIL-2R的水平较治疗前降低,但无统计学差异(P>0.05)。结论:HIFU具有免疫激活作用,可一定程度改善患者的机体免疫功能。Objective：To study the changes of the immune function in metastatic hepatic carcinoma patients after high intensity focused ultrasound treatment. Methods：Under general anaesthesia and ultrasound positioning, thirty metastatic hepatic carcinoma patients were treated by high intensity focused ultrasound. Flow cytometry （FCM） was used to detect the changes in percentages of T - lymphocyte subsets （ CD3＋ , CD4＋ , CD8＋ ）, NK cells and the contents of soluble interleukin-2 receptor （sIL- 2R） were detected by double sandwich ELISA before and after HIFU treat- ment. LDH enzyme -release assay was used to detect cytotoxic activity of NK cells. Results： After HIFU treatment, the percentages of CD3^＋ and CD4^＋ and NK cell：s significantly increased （P 〈 0.05 ）, the percentage of CD8＋ cells decreased markedly （ P 〈 0.05 ）. By comparison with pre - therapy group, the cytotoxic activity of NK cells increased and the content of sIL- 2R decreased, but they both were no statistical difference between post -therapy and pre - therapy （ P 〉 0.05 ）. Conclusion： High intensity focused ultrasound may activate and improve cell - mediated immune function in a certain degree.

关 键 词：转移性肝癌 高强度聚焦超声 T细胞亚群 NK细胞 NK细胞杀伤活性 可溶性白细胞介素-2受体 (sIL-2R) 

分 类 号：R735.7[医药卫生—肿瘤]