釉基质蛋白对化学处理钛表面磷灰石生成的影响  

Effect of enamel matrix proteins on the growth of apatite coating on chemically modified titanium

作　　者：王晓洁[1] 黄慧[1] 杨斐 张富强[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海市口腔医学重点实验室,上海200011 [2]上海市口腔病防治院,上海200031

出　　处：《上海口腔医学》2010年第1期49-54,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30670555);上海市重点学科建设项目(S30206);上海市科学技术委员会资助项目(08DZ2271100)~~

摘　　要：目的:研究釉基质蛋白对双重热酸蚀及碱热处理纯钛表面仿生矿化沉积磷灰石层的影响。方法:提取猪未萌恒牙胚表面EMPs,用SDS-PAGE电泳进行验证。纯钛片经抛光清洗,分别进行双重热酸蚀和碱热处理,放入含EMPs(150μg/mL)的改良模拟体液(m-SBF)中浸泡7d,对照组为不含EMPs的m-SBF。扫描电镜观察试件表面形貌,X射线能谱,X射线衍射及傅里叶红外光谱等分析其元素成分及晶相结构。结果:酸蚀组经仿生矿化,对照组试件表面无沉积物生成,实验组加入EMPs,钛片表面有一定量的沉积物生成,能谱分析显示主要由Ca、P、O和C等元素组成。碱热处理组经仿生矿化,对照组与实验组试件表面均有沉积层形成,但后者有较多直径约为300～600nm的孔隙生成,元素组成主要为Ca、P、O和C,X射线衍射及傅里叶红外光谱分析显示沉积物为碳羟基磷灰石。结论:碱热处理纯钛表面有利于磷灰石层的形成,加入EMPs能促进磷灰石层的形成并改变其形貌。PURPOSE： To evaluate the effects of enamel matrix proteins（EMPs） on the growth of apatite coatings on dual thermo-etching or alkali heat-treated pure titanium（Ti）. METHODS： EMPs were extracted from porcine tooth germs and identified by SDS-PAGE. Dual thermo-etching treated or alkali-heat treated Ti samples, then, were exposed in a blank or EMPs containing（150μg/mL） modified simulated body fluid（m-SBF） at 37℃ for 7 days. SEM, EDS, XRD and FTIR were employed to observe or analyze the morphologies, element components and crystal structures of the apatite coatings. RESULTS： In dual thermo-etching-treated groups, almost no calcium-phosphate deposits were found on the titanium surfaces of control group and some amount of calcium-phosphate deposits with the main element components of Ca, P, O and C, however, formed on those of experimental group. But in alkali heat-treated groups, the apatite coatings proved to be carbonate hydroxyapatite formed on the titanium surfaces of both control group and experimental group, and the apatite coatings of the latter were found porous（300-600nm）and uneven. CONCLUSIONS： Alkali heat-treatment is in favor of apatite deposition, and EMPs can promote apatite deposition on both dual thermo-etching and alkali heat-treated pure titanium surfaces.

关 键 词：釉基质蛋白 纯钛 模拟体液 磷灰石 

分 类 号：R783.1[医药卫生—口腔医学]