口腔疣状癌伴灶性鳞癌颈淋巴结转移灶裸鼠移植瘤模型的建立  

Establishment of xenografted model of oral verrucous carcinoma with focal squamous cell carcinoma metastasizing to the cervical lymph nodes in nude mice

作　　者：王月红[1] 陈明伟[1,2] 唐瞻贵[1] 刘欧胜[1] 方小丹[1] 全宏志[1] 

机构地区：[1]中南大学湘雅医院.口腔医学院口腔颌面外科,湖南长沙410078 [2]山东省济宁市第一人民医院口腔科,山东济宁272000

出　　处：《上海口腔医学》2010年第1期55-59,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30872895);湖南省科技厅科技计划重点项目(2008FJ2011);湖南省博士后基金(2006FJ4246)~~

摘　　要：目的:建立口腔疣状癌伴灶性鳞癌颈淋巴结转移灶移植瘤原代模型,探讨建立模型的方法。方法:将口腔疣状癌伴灶性鳞癌颈淋巴结转移组织,通过组织块皮下接种法建立颈部淋巴结转移灶模型。观察其生长状况,对移植瘤、裸鼠肝、肾、肺及淋巴结行系列检查并与来源肿瘤组织进行比较。结果:成瘤率87.5%(7/8),移植瘤具有来源肿瘤组织类似的形态学特征,肝、肾、肺未见转移,腋下及腹股沟淋巴结反应性增生。结论:通过组织块皮下接种法,成功建立了口腔疣状癌伴灶性鳞癌颈淋巴结转移灶移植瘤原代模型。PURPOSE： To use the excised cervical lymph node tissue from oral verrucous carcinoma patient with focal squamous cell carcinoma subcutaneously to establish a xenografted model in nude mice. METHODS： The xenograft tumors were finally removed for histopathological study and the mice were laparotomized to examine metastatic tumors in livers, kidneys, lungs. RESULTS： The tumor formation rate was 87.5%（7/8）,and the appearance of transplanted tumors was like that in human and HE staining showed that the cancer cells of those tumors models and mesenchymal components remained morphologically like the original tumor. The liver, renal, lung and lymph nodes didn’t show obvious metastasis. CONCLUSION： The xenografted model is successfully established with a higher formation rate, and the model morphologically resembles the human tumor.

关 键 词：口腔疣状癌 移植瘤 裸鼠 动物模型 

分 类 号：R739.8[医药卫生—肿瘤]