3种临床常用烤瓷合金对小鼠成纤维细胞L929的毒性评价  

Study on cytotoxicity of three kinds of dental ceramic alloys on L929 mouse fibroblasts in vitro

作　　者：乔广艳[1,2] 沈庆平 苏俭生[2] 

机构地区：[1]上海市口腔病防治院修复科,上海200001 [2]同济大学口腔医学院修复科,上海200072

出　　处：《上海口腔医学》2010年第1期72-76,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30970726)~~

摘　　要：目的:评价3种临床常用牙科烤瓷合金材料的细胞毒性。方法:采用一种能快速评定细胞增殖率和细胞毒性的CCK-8比色法,检测镍铬合金(Ni,77.36%)、钴铬合金(Co,61.0%)、金合金(Au,58.0%)等3种烤瓷合金材料对小鼠成纤维细胞L929相对增殖率的影响;应用单细胞凝胶电泳(彗星电泳),检测3种烤瓷合金材料对小鼠成纤维细胞L929 DNA的损伤情况。采用SAS9.0软件包对数据进行统计学分析。结果:镍铬合金组、钴铬合金组和金合金组细胞的相对增殖率分别为(75.9510±7.6244)%、(84.8920±8.2660)%和(88.5420±12.3611)%。镍铬合金组的细胞毒性显著高于钴铬合金组(P<0.05)和金合金组(P<0.05),而钴铬合金组与金合金组之间无显著差异(P>0.05),但3种材料的细胞毒性分级均为1级,表现为轻微毒性;镍铬合金组DNA损伤程度的彗星数目显著高于钴铬合金组(P<0.05)和金合金组(P<0.05),而钴铬合金组与金合金组无显著差异(P>0.05)。结论:钴铬烤瓷合金材料的细胞毒性显著低于镍铬烤瓷合金材料,而与58%金烤瓷合金材料的细胞毒性接近。临床上应尽可能选择钴铬烤瓷合金或58%金烤瓷合金等细胞毒性较低的烤瓷合金材料。PURPOSE： To investigate the cytotoxicity in vitro of three kinds of dental ceramic alloys on L929 mouse fibroblasts. METHODS： CCK-8 assay was a quick method to study the cell relative proliferation rate or cytotoxicity. L929 mouse fibroblasts were used to detect the cell relative proliferation rate of the three kinds of dental ceramic alloys （NiCr,CoCr and golden alloys containing 58% of gold） by CCK-8 assay. The DNA damage of L929 mouse fibroblasts was determined by single cell gel electrophoresis （SCGE）. The data was statistically analyzed with SAS 9.0 software package. RESULTS： The relative growth rate induced by NiCr, CoCr and golden dental ceramic alloys was （75.9510±7.6244）%, （84.8920±8.2660）% and （88.5420±12.3611）%, respectively. NiCr ceramic alloys caused the most marked cytotoxicity（P 〈0.05）, and there was no difference between CoCr ceramic alloys and 58% golden ceramic alloys（P〉0.05）. The cytotoxicity of the three kinds of metal ceramic alloys was all grade 1, i.e. mild cytotoxicity. The DNA damage of L929 mouse fibroblasts induced by NiCr ceramic alloys was most serious （P〈0.05）. There was no difference in the DNA damage between CoCr ceramic alloys and 58% golden ceramic alloys （P〉0.05）. CONCLUSIONS： NiCr ceramic alloys can suppress the proliferation of the L929 mouse fibroblasts and promote DNA damage. While CoCr ceramic alloys which are similar to 58% golden ceramic alloys show less cytotoxicity than NiCr ceramic alloys. CoCr or 58% golden ceramic alloys should be used as crown and bridge for patients instead of NiCr ceramic alloy clinically.

关 键 词：牙科烤瓷合金 细胞毒性 CCK-8 小鼠成纤维细胞L929 DNA 彗星电泳 

分 类 号：R783.1[医药卫生—口腔医学]