儿茶素对大鼠脑缺血/再灌注损伤的保护作用及机制  

Protective effects of catechin on cerebral ischemia-reperfusion injury in rats and its mechanism

作　　者：刘珊丽[1] 刘宗文[2] 卢沛琦[3] 张艳[1] 张建东[1] 贾丹辉[1] 姚昱欧[1] 曹志斌[1] 

机构地区：[1]郑州大学基础医学院药理学教研室,河南郑州450001 [2]郑州大学第二附属医院肿瘤科,河南郑州450003 [3]郑州大学第一附属医院神经内科,河南郑州450052

出　　处：《中国药理学通报》2010年第2期255-257,共3页Chinese Pharmacological Bulletin

基　　金：河南省自然科学基础研究资助项目(No0511040800)

摘　　要：目的探讨儿茶素对大鼠脑缺血/再灌注损伤的保护作用及机制。方法40只大鼠随机分成5组,假手术组、模型组和儿茶素低、中、高剂量(50,100,200mg·kg-1)组。采用改良的线栓法制备大鼠局灶性脑缺血(2h)/再灌注(24h)损伤模型,儿茶素各组于缺血前30min和缺血后2h腹腔注射儿茶素溶液,假手术组和模型组给与等体积的NS。再灌注24h,用改良的Bederson评分法对大鼠进行神经功能缺损评分后,进行海马组织形态学观察及MPO和NOS活性测定。结果儿茶素可降低大鼠脑缺血/再灌注损伤的神经功能缺损评分,能不同程度地改善脑组织学损伤,并且降低脑组织中MPO和NOS的活性。结论儿茶素对大鼠脑缺血/再灌注损伤有保护作用,作用机制之一可能与抗炎、抗氧化有关。Aim To investigate the protective effect of catechin on cerebral ischemia-reperfusion injury in rats and its mechanism. Methods 40 rats were randomly divided into 5 groups： sham operation group, model group and 50,100 and 200 mg · kg^-1 catechin groups, with 8 rats in each group. The model of focal cerebral ischemia-reperfusion in rats was established with modified sutured-occluded method. The rats in catechin groups were injected with catechin at the matched concentration. The rats in sham operation group and model group were injected with saline. And all rats were given more time in 2 hours after ischemia. Rats were sacrificed for histologic examination after the behavioraltest, and their brains were taken to assay the activities of MPO and NOS. Results ges（50,100 and 200 mg Catechin at different dosages（50,100 and 200 mg·kg^-1）could obviously decrease neurological deficit score, repair histological injury, and reduce the activities of MPO and NOS in rats of focal cerebral ischemia-reperfusion injury. Conclusions Catechin can relieve the cerebral ischemia reperfusion injury, and its mechanism may be partly related to the effects of its antiinflammation and antioxidation.

关 键 词：脑缺血/再灌注 保护作用 儿茶素 抗炎 抗氧化 髓过氧化物酶 一氧化氮合酶 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]