猫眼草水煎液体外致突变性的研究  

Mutagenicity of Enphorbia lunulata decoction in vitro

作　　者：金建玲[1] 张辉[1] 刘波[1] 蔡玉品[1] 高培基[1] 

机构地区：[1]山东大学生命科学学院微生物技术国家重点实验室,山东济南250100

出　　处：《中国药理学通报》2010年第2期263-266,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No39430020)

摘　　要：目的检验猫眼草水煎液的致突变性;改进经典的A-mes试验体系使之适应于中药体外致突变性检验。方法通过经典的Ames试验检测猫眼草的体外致突变性;通过哺乳动物骨髓细胞染色体畸变试验检测猫眼草的致畸作用;改进的Ames试验通过增设含补充组氨酸(含量对应于猫眼草水煎液中组氨酸浓度)的阴性对照,排除样品中组氨酸成分对试验结果的影响。结果猫眼草水煎液在经典的Ames试验中为强阳性;对哺乳动物骨髓细胞染色体致畸作用为阴性;在改进的Ames试验中猫眼草水煎液的致突变性为阴性。结论经典的Ames试验不适合猫眼草水煎液致突变性检测,改进后的Ames实验体系适合。猫眼草水煎液在体外和体内均没有致突变性。Aim To assay the mutagenicity of Enphorbia lunulata （EL） decoction and to modify the Ames test for evaluation the mutagenicity of herbal medicine samples. Method The mutagenicity of EL decoction was assayed by standard Ames test; the teratogenieity was done by mammalian bone marrow chromosomal aberration test. In modified Ames test system, the influence of histidine EL decoction was excluded by additional negative control, in which the test media was supplied with histidine （histidine amount equaled to the histidine in different concentration of EL decoction）. Result The mutagenicity of EL decoction waspositive in the standard Ames test. The teratogenicity of EL decoction was negative in mammalian bone mar- row chromosomal aberration test. By the modified Ames tests, the mutagenicity of EL decoction was negative. Conclusion The standard Ames test is not suitable for evaluating the mutagenicity of EL decoction, but the modified Ames test is. The mutagenicity in vitro and the teratogenicity in vivo of EL decoction are all negative.

关 键 词：猫眼草 AMES试验 致突变性 染色体畸变 遗传毒性 国家标准 

分 类 号：R332[医药卫生—人体生理学] R282.710.5[医药卫生—基础医学]