以淀粉为碳源衣康酸高产菌株的筛选  

Screening of High Itaconic Acid Yielding Strain Using Corn Starch

作　　者：魏凌云 梁海秋[2] 周河治[2] 张佑红[1] 丁一刚[1] 

机构地区：[1]武汉工程大学绿色化工过程省部共建教育部重点实验室,湖北武汉430073 [2]广西大学生命科学与技术学院,广西南宁530004

出　　处：《微生物学通报》2010年第2期246-250,共5页Microbiology China

基　　金：湖北省教育厅科研项目(No.B20081509)

摘　　要：对土曲霉出发菌株进行紫外线诱变、LiCl诱变以及代谢终产物抗性菌株选育。代谢终产物抗性菌株选育是一种有效的遗传育种方法,能显著提高产酸量。得到一株代号为At394的菌株,以玉米淀粉部分水解糖为碳源,产酸量为53.9g/L,比出发菌株提高了42.6%。糖酸转化率为61.5%,为所有筛选菌株最高。用红外光谱进行结构分析证实所得产物为衣康酸。This paper described the screening of itaconic acid strain of Aspergillus terreus with UV-irradiation, LiCl treatment and breeding resistant strain to metabolic end product. Breeding resistant strain was an effective measure to help raise yields steadily. A strain, designated At394, producing itaconic acid with a high yield was successfully obtained. The itaconic acid concentration produced by At394 was 53.9 g/L using starch hydrolysate in shaking flasks, which was 42.6% higher than that of the parental strain. The conversion rate was 61.5%, which was the highest value found in tests. The structure determination by infrared spectrum showed the production obtained was itaconic acid.

关 键 词：衣康酸 土曲霉 玉米淀粉 筛选 

分 类 号：Q933[生物学—微生物学]