流动注射氢化物发生原子吸收光谱法测定禽蛋中的硒  

Determination of Selenium in Poultry Eggs by Flow Injection-Hydride Generation-Atomic Absorption Spectrometry

作　　者：邓世林[1] 李新凤[1] 郭小林[1] 

机构地区：[1]中南大学现代分析测试中心,湖南长沙410078

出　　处：《光谱学与光谱分析》2010年第3期809-811,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30170852)资助

摘　　要：探讨了流动注射-氢化物发生-原子吸收光谱法测定硒时的最佳条件,建立了禽蛋中微量硒的流动注射-氢化物发生-原子吸收光谱分析方法。同时讨论了禽蛋中硒含量水平和富硒科学饲养提高禽蛋中硒含量在补硒食品中的发展前景。在优化的工作条件下,测定硒的检出限为0.25μg.L-1,线性范围0~60μg.L-1,相对标准偏差小于2.5%,加标回收率为95%~108%。此法克服了石墨炉法存在严重的基体干扰,需要加入适当基体改进剂以提高硒的灰化温度及传统间断氢化物发生-原子吸收光谱法分析速度慢、操作繁琐且手工进样带来的误差等缺点,操作简便、快速、灵敏度和自动化程度高。A flow injection- hydride generation-atomic absorption spectrometric method for the determination of selenium in poultry eggs was developed. Various testing conditions and effect factors for the determination of selenium were studied. The authors also discussed the content of selenium in poultry eggs and the application of scientific feed method in improving selenium content in poultry eggs. The detection limit for selenium was 0. 25 μg · L^-1 under optimum conditions. The linear range for selenium was 0. 25-60 μg · L^-1. The relative standard deviation was less than 2.5% and the recovery was 95%-108%. This method overcame the problem of severe matrix interferences of graphite furnace atomic absorption spectrometry, in which matrix modifier must be added to eliminate matrix interference by raising ashing temperature, Additionally this method overcame the shortcoming of slow analysis procedure, complex operation and error caused by manual injection in traditional interval hydride generation-a- tomic absorption spectrometry. It is a simple, rapid, highly sensitive and automatic method and has been applied to the determination of selenium in poultry eggs with satisfactory results.

关 键 词：流动注射氢化物发生-原子吸收光谱法 禽蛋 硒 含量测定 

分 类 号：O657.3[理学—分析化学]