rhIL-2体外激活骨髓细胞产生TNF-α与激活骨髓抗肿瘤活性的关系研究  

TNF-α production and antitumor effect of rhIL-2-activated bone marrow cells

作　　者：朱步海[1] 杨平地[1] 孙秉中[1] 朱华锋[1] 于文强[1] 

机构地区：[1]第四军医大学西京医院血液科,西安710032

出　　处：《中华血液学杂志》1998年第11期581-582,共2页Chinese Journal of Hematology

摘　　要：目的:进一步探讨重组人白细胞介素2(rhIL-2)体外激活骨髓细胞产生细胞毒作用的机制。方法:取人新鲜骨髓中单个核细胞,体外与rhIL-2孵育24小时及48小时,即为激活的骨髓(ABM),同时设不加rhIL-2组(NBM)为对照。分别检测ABM和NBM的细胞毒活性、培养液中肿瘤坏死因子α(TNF-α)水平,并进行比较观察和相关性分析。结果:ABM对HL-60细胞的细胞毒活性较NBM明显增强,培养液中TNF-α水平明显增高(P<0.05),且ABM的TNF-α水平与同时相的细胞毒活性呈密切正相关。结论:rhIL-2激活骨髓细胞产生的TNF-α参与ABM的肿瘤杀伤活性。Objective: To explore the mechanism of the cytotoxic effects of bone marrow cells activated with recombinant human interleukin-2(rhIL-2). Methods: The cytotoxicity of rhIL-2-activated bone marrow (ABM) and the TNF-α level in the supernant of ABM culture were measured after activated for 24 and 48 hours. Results: The cytotoxic activity against HL-60 cells and the level of TNF-α were significantly increased in ABM as compared with that in non-ABM(P<0.01). Meanwhile, there was a significantly positive relationship between the TNF-α levels in supernant and cytotoxicities of ABM. Conclusion: TNF-α produced by rhIL-2 acti- vated bone marrow cells involved in the antitumor effect of ABM.

关 键 词：RHIL-2 骨髓细胞 激活 肿瘤坏死因子Α 肿瘤 

分 类 号：R730.51[医药卫生—肿瘤]