原发性肝癌患者血清和肝组织中庚型肝炎病毒基因的PCR检测  

Detection of Hepatitis G Virus Sequence in Sera and Liver tissues from Patients with Primary Liver Cancer

作　　者：崔晓红[1] 戚中田[1] 潘卫[1] 陈景山[1] 宋艳斌[1] 朱诗应[1] TerukatsuArim 

机构地区：[1]第二军医大学微生物学教研室,200433 [2]日本鹿儿岛大学医学院

出　　处：《临床肝胆病杂志》1998年第4期217-219,共3页Journal of Clinical Hepatology

基　　金：国家自然科学基金!39680018

摘　　要：用逆转录－聚合酶链反应法（RT—PCR）检测了原发性肝癌（PLC）患者血清及肝癌和癌旁肝组织中的庚型肝炎病毒（HGV）RNA，以PCR－双脱氧末端终止法测定了PCR产物的核苷酸序列。结果显示，血清和肝组织中HGVRNA的检出率分别为19．4％（13／67）和25．7％（9／35），且HGVRNA在肝癌组织和癌旁肝组织中同时存在；血清和肝组织中HGVRNA检测结果的符合率为85％；5非编码区（5＇－NCR）扩增片段的核着酸序列与美国株GBV—C的同源性为91．7％。本研究结果提示HGV感染在我国PLC患者中存在，且血清HGVRNA的检测能反映HGV在肝内的复制情况。Serum and liver samples from patients with primary liver cancer (PLC) were examined for the presence of heptitis G virus HGV RNA by reverse transcription-polymerase chainreaction (RT-PCR) using primers from HGV 5' non-coding region (51NCR). The nucleotide sequences of the PCR products were determined directly by dideoxynucleotide chain termination-PCRmethod. The positive rates of HGV RNA in sera and liver tissue were 19. 4% (13/67) and 25. 7%(9/35),respectively. HGV RNA was detected in cancerous and non-cancerous tissues simultaneously. The identical rate of HGV RNA in serum and liver was 85%. The 51NCR sequences of thePCR products showed 91. 7% of homology in comparison with GBV-C strain. It is suggested thatHGV infection exists in patients with PLC in our country,and the detection of HGV RNA in serummay reflect HGV replication in liver.

关 键 词：肝癌 庚型肝炎病毒 聚合酶链反应 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]