大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究  

Effect of MAPKK/MEK-MAPK/ERK Signal Transduction Pathway in Rat Neural Stem Cells

作　　者：赵宇[1] 谢鹏[1] 朱晓峰[2] 蔡志友[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]佳木斯大学神经科学研究所,黑龙江佳木斯154007

出　　处：《中国医科大学学报》2010年第1期14-17,共4页Journal of China Medical University

基　　金：高等学校博士学科点专项科研基金资助项目(20050631012)

摘　　要：目的应用丝裂原激活的蛋白激酶激酶(MAPKK/MEK)特异性阻滞剂PD98059孵育大鼠神经干细胞(NSC),探讨MAPKK/MEK-MAPK/ERK信号通路在NSC中的作用。方法体外分离、培养大鼠NSC,应用不同浓度的PD98059孵育NSC后进行WTS-8、Nestin,BrdU、β-tubulin-Ⅲ、Western blot检测。结果PD98059对NSC的存活、增殖和分化的影响呈剂量依赖性,在PD98059较高浓度时NSC的存活率明显下降(P<0.05),BrdU和β-tubulin-Ⅲ的阳性细胞数明显少于正常对照组(P<0.05)。PD98059阻断ERK表达的作用呈剂量依赖性。结论MAPKK/MEK-MAPK/ERK信号通路对大鼠NSC的存活、增殖、分化起重要作用。Objective To incubate the rat neural stem cells with specific inhibitor（PD98059）of MAPKK/MEK to clarify the effect of MAPKK/MEK-MAPK/ERK signaling pathway in neural stem cells （NSC）. Methods NSCs derived from E15-16 rats were isolated and cultured. After treated with different concentration of PD98059,they were subjected to the detection of cell proliferation,Nestin,BrdU and β- tubulin-Ⅲ by WTS-8 assay,immunofluorescent staining or Western Blot respectively. Results PD98059 had an effect on the survival,proliferation and differentiation of NSC at a concentration-dependent manner. The viability of the NSC was significantly decreased than the control（P 〈 0.05）,whereas the numbers of BrdU-positive and β-tubulin-Ⅲ positive cells were notably fewer than the control（P 〈 0.05）after incubated with the higher concentration of PD98059. The ERK expression was blocked by PD98059 at a concentration-dependent manner. Conclusion The MAPKK/MEK-MAPK/ERK signaling pathway played a vital role in the survival,proliferation and differentiation of NSC.

关 键 词：丝裂原激活的蛋白激酶激酶 细胞外信号调控激酶 神经干细胞 

分 类 号：R742[医药卫生—神经病学与精神病学]