基于人HMGB1-B box的抑制性小肽筛选及其抗炎功能分析  

Screening of Small Inhibitive Peptides Binding Human HMGB1-B Box and Characterization of their Anti-inflammative Effects

作　　者：许志臻[1] 黄刚[1] 樊继山[1] 曾益军[1] 张艳[1] 陈岑曦[1] 何凤田[1] 

机构地区：[1]第三军医大学基础部生物化学与分子生物学教研室,重庆市400038

出　　处：《医学分子生物学杂志》2010年第1期11-15,共5页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.30670411）,军队“十一五”医药卫生研究基金资助项目（No.06H027）,重庆市自然科学基金（No.CSCT2008BB5022）

摘　　要：目的从噬菌体构象型7肽库中筛选人HMGB1-Bbox的抑制性小肽。方法以重组人HMGB1-Bbox为靶分子对噬菌体构象型7肽库进行6轮亲和筛选，获得Bbox结合的克隆，并经ELISA验证。选取亲和力高的克隆进行DNA测序，并推导出呈现的多肽序列，通过IL-6 ELISA检测噬菌体呈现的小肽对人HMGB1-B box致炎功能的抑制作用。结果经过6轮亲和筛选，噬菌体的回收率增加，阳性克隆得到富集。挑选15个结合力强的克隆进行测序，推导出2个多肽序列。所获两个阳性噬菌体克隆能特异性地抑制人HMGB1-B box刺激THP-1细胞产生炎症因子的能力。结论获得了噬菌体呈现的能够抑制人HMGB1-B box的两个小肽。Objective To screen and characterize antagonistic peptides inhibiting hHMGB1-B box. Methods C7C phage display peptide library was screened with hHMGB1-B box. Positive phage clones were identified by ELISA. Results After 6 rounds of screening, the ratio of output to input and specific enrichment was gradually increased and positive clones were enriched. Two phage clones inhibiting the activity of HMGB1-B box were identified. Conclusion Two phage display antagonist peptides blocking HMGB1-B box were obtained.

关 键 词：HMGB1-B BOX 噬菌体肽库 小肽 

分 类 号：Q514[生物学—生物化学] Q782