NHE1反义基因对胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响  

Effect of Antisense gene NHE1 on the expression of NHE1 protein of gastric cancer cell after transfection

作　　者：滕小春[1] 刘海峰[2] 

机构地区：[1]重钢总医院老年科,重庆400081 [2]北京武警总医院消化科,北京100039

出　　处：《重庆医科大学学报》2010年第1期57-59,共3页Journal of Chongqing Medical University

摘　　要：目的:探讨人NHE1反义基因转染对SGC-7901胃癌细胞NHE1蛋白表达及裸鼠成瘤力的影响。方法:构建人NHE1反义基因真核表达载体,采用脂质体法将其转染至SGC-7901胃癌细胞中,通过免疫组织细胞化学SP法检测NHE1蛋白表达情况,流式细胞仪检测对细胞增殖和凋亡的影响,从电镜水平比较观察转染与未转染细胞形态学的变化,并观察裸鼠成瘤能力的改变。结果:转染NHE1反义基因的SGC-7901胃癌细胞NHE1蛋白表达降低;细胞增殖能力下降,细胞凋亡率增加;透射电镜下细胞线粒体数量增多、体积增大,内质网扩张,核偏向一侧,极性增强;裸鼠成瘤能力下降。结论:NHE1反义基因能使SGC-7901胃癌细胞的NHE1蛋白表达明显下调,裸鼠成瘤能力降低,NHE1蛋白在胃癌细胞生长中起重要作用。Objective： To study the effects of antisense human NHE1 gene on the expression of the NHE1 protein of SGC-7901 gastric cancer cell and the changes of the carcinoma forming capacity after transplantation in nude mouse. Methods Antisense NHEI euka~,otic expression vector was constructed with recombinant DNA technique and transfected into the cells of gastric carcinoma cell line SGC-7901 with liposome DOTAP.Then,the expression of NHE1 protein with SP and the changes of the carcinoma forming capacity in nude mouse was also observed. Results： The expressions of NHE1 protein of SGC-7901 cells transfecled with antisense NHE1 gene in vitro were obviously decreased, inhibitions of their proliferation were induced, apoptosis rate increased.Under electron microscope, organelles in these cells were abundant, carcinoma forming capacity in nude mouse were decreased. Conclusion：The expression of NHE1 protein was obviously down regulated after the transfection of antisense NHE1 gene,and carcinoma forming capacity in nude mouse was decreased. which implies that NHE 1 protein plays an important role in gastric cancer cell growth.

关 键 词：NHE1基因 真核表达载体 反义基因 胃癌 

分 类 号：R734.2[医药卫生—肿瘤]