急性淋巴细胞白血病患儿血清CD105、血管内皮生长因子及其受体的测定和意义  

Detection and clinical implications of serum CD105,vascular endothelial growth factor and its receptor in acute lymphoblastic leukemia children

作　　者：黄婕[1] 方拥军[1] 施圣云[1] 张兰芳[1] 陆世丰[1] 陆勤[1] 何璐璐[1] 芮耀耀[1] 杨引[1] 周莉[1] 吴鹏[1] 

机构地区：[1]南京医科大学附属南京儿童医院血液肿瘤科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2010年第2期239-241,248,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金(06NMUM043)

摘　　要：目的:探讨急性淋巴细胞白血病(ALL)患儿血清CD105、血管内皮生长因子(VEGF)及其受体Flt-1的水平和临床意义。方法:用双抗体夹心酶免疫吸附法(ELISA)检测31例初诊ALL患儿及10例健康对照组儿童血清CD105、VEGF及其受体Flt-1水平。结果:初诊ALL患儿血清VEGF及Flt-1水平与对照组无显著差异,而CD105水平明显高于对照组(P<0.01)。高危组ALL患儿CD105水平明显高于低危组(P<0.05)。CD105水平与患儿性别、年龄、初诊白细胞计数及免疫分型有无髓系抗原表达等因素无关,染色体核型正常与异常组间也无显著差异,但在有不同的肝脾淋巴结等髓外浸润表现及不同的早期治疗反应的患儿间差异有统计学意义(P<0.05)。结论:初诊ALL患儿VEGF及Flt-1水平与健康对照组无显著差异。血浆CD105水平有助于临床指导治疗和判断预后。Objective：To investigate the level and clinical implications of serum CD105,vascular endothelial growth factor （VEGF） and its receptor Flt-1 in acute lymphoblast leukemia （ALL） children. Methods：Serum levels of CD105,VEGF and Flt-1 were detected in 31 newly diagnosed ALL children and 10 health controls with double antibodies sandwich enzymelinked immurosorbent assay. Results：The serum levels of VEGF and Flt-1 were not significantly different between the newly diagnosed ALL children and the health controls. While the serum level of CD105 was significantly higher in the ALL children than that in the health controls （P 〈 0.01）,and the level was significantly higher in the high risk ALL children than in the low risk group （P 〈 0.05）. The serum level of CD105 was independent of sex,age,white blood cell count when diagnosed,and immunophenotype with or without myeloid antigens, but it was related to extramedullary manifestations such as enlargement of live,spleen and lymph nodes,and ear...更多ly treatment response （P 〈 0.05）. The serum level of CD105 was not significantly different between ALL children with chromosome abnormality and without abnormality. Conclusion：There was no significant difference in the serum levels of VEGF and Flt-1 between the ALL children and the health controls. The serum level of CD105 might be helpful in guiding therapy and evaluating prognosis.

关 键 词：白血病 血管内皮生长因子 CD105 

分 类 号：R733.71[医药卫生—肿瘤]