槲皮素对大肠癌细胞SW-480增殖、侵袭力及其表面抗原-2表达的影响  

Effects of quercetin on proliferation,invasiveness and expression of trophoblast cell-surface antigen-2 of human colorectal cancer cell SW-480

作　　者：钟锡明[1] 范钰[1] 王崇强[1] 邱志远[1] 黄卫兵[1] 

机构地区：[1]江苏大学附属人民医院,江苏镇江212002

出　　处：《山东医药》2010年第10期1-2,共2页Shandong Medical Journal

基　　金：江苏省自然科学基金资助项目(2009205);镇江市社会发展基金资助项目(SH2009014)

摘　　要：目的观察槲皮素(Que)对大肠癌细胞增殖、侵袭力的影响,并探讨其可能机制。方法加入不同浓度的Que培养大肠癌细胞SW-480后,用软琼脂克隆培养试验检测SW-480的增殖能力,用Boyden小室模型法检测SW-480侵袭力,用荧光实时定量RT-PCR检测SW-480的滋养层细胞表面抗原2(TROP-2)mRNA,以Western blot法检测SW-480的TROP-2蛋白。结果SW-480加入Que培养后,恶性增殖和穿膜细胞数均明显下降,且呈剂量依赖性(P均<0.01);同时其TROP-2 mRNA和蛋白表达水平均明显下调,且呈时间和剂量依赖性(P均<0.01)。结论Que可明显抑制大肠癌SW-480细胞的增殖、侵袭力,其机制可能与下调TROP-2基因表达有关。Objective To study the effects and mechanism of quereetin on human eoloreetal cancer cell, Methods Human eolorectal cancer cells SW-480 were treated with different doses of quereetin, and the growth of anchorage independence of SW-480 was studied by colony formation in soft agar, the invasiveness of SW-480 was determined by Boyden chamber, the expression of mRNA and protein of trophoblast cell-surface antigen-2 （TROP-2）gene were detected by real time RT-PCR and Western blot assay, respectively. Results Transmembrane cell and anchorage independence growth of querce- tin group decreased in dose-dependent manners（P 〈0.01 ）. The level of mRNA and protein of TROP-2 of SW-480 treated with quercetin reduced in time- and dose-dependent manners（P 〈0.01 ）. Conclusions Quercetin can inhibit invasiveness and proliferation of human colorectal carcinoma cells, and decreasing the expression of TROP-2 gene may play an important role in its mechanism.

关 键 词：大肠肿瘤 槲皮素 细胞增殖 细胞侵袭力 滋养层细胞表面抗原 

分 类 号：R735.2[医药卫生—肿瘤]