膈下逐瘀汤联合环磷酰胺诱导Hca-f肝癌小鼠细胞调亡的实验研究  

Experimental Study of Decoction for Removing Blood Stasis in Diaphragm Combined with Cyclophosphamide on Inducing Apoptosis of Hca-f Liver Cancer Mice

作　　者：连媛媛[1] 赵金茹[1] 任蕊[1] 刘春英[1] 

机构地区：[1]辽宁中医药大学

出　　处：《山西中医》2010年第3期50-51,56,共3页Shanxi Journal of Traditional Chinese Medicine

摘　　要：目的:研究膈下逐瘀汤联合环磷酰胺(CTX)对Hca-f肝癌小鼠的抑瘤作用及其机制。方法:无菌条件下抽取Hca-f肝癌小鼠腹水,予生理盐水稀释,调细胞浓度为2×107个瘤细胞/mL,分别接种于每只小鼠右腋下皮部0.2 mL,24 h后随机分成模型组、CTX组、中药组、中药+CTX组,每组10只,给药8天后处死小鼠,剥离并称量瘤重计算抑瘤率。光镜下观察肿瘤细胞形态变化,电镜下观察凋亡小体。结果:膈下逐瘀汤联合环磷酰胺抑瘤率达到47.75%,能够有效诱导肿瘤细胞凋亡。结论:膈下逐瘀汤联合环磷酰胺对Hca-f肝癌小鼠有明显的抑瘤作用,其抑制肿瘤的可能机制是诱导肿瘤细胞凋亡。Objective：To study the inhibiting-tumor effect of Decoction for Removing Blood Stasis in Diaphragm combined with Cyclophosphamide （CTX） on Hca-f liver cancer mice and its mechanism. Method ： Under sterile conditions, collect ascites of each Hca-f liver cancer mouse, dilute with normal saline, adjust the cell concentration of 2 × 10^7 tumor cells/mL, and inoculate 0.2 mL in each mouse in the right axilla skin. After 24 h the mice were randomly divided into model group, CTX group, the Chinese medicine group, Chinese medicine and CTX group, ten mice in each group. Eight days after administration the mice were killed, stripped and weighed tumor to calculate inhibitory rate of tumor. Observe morphological changes of tumor cells under light microscope and apoptotic bodies under electron microscope. Result：Decoction for Removing Blood Stasis in Diaphragm combined with Cyclophosphamide could effectively induce apoptosis of tumor cells, and the inhibitory rate of tumor reached 47.75%. Conclusion： Decoction for Removing Blood Stasis in Diaphragm combined with Cyclophosphamide has marked effect on inhibiting tumor growth of Hca-f Hca-f liver cancer mouse, and the possible mechanism is to induce apoptosis of tumor cells.

关 键 词：膈下逐瘀汤 环磷酰胺 Hca—f 抑瘤率 实验研究 

分 类 号：R735.7[医药卫生—肿瘤] R285.5[医药卫生—临床医学]