大鼠再生肝8种细胞的脂肪酸代谢基因转录谱预示的生化活动  

Expression Profiles of Fatty Acid Metabolism-Associated Genes Uncovered Biochemical Activities in 8 Liver Cell Types during Rat Liver Regeneration

作　　者：郭学强[1] 秦波[1,2] 徐存拴 

机构地区：[1]河南省-科技部共建细胞分化国家重点实验室培育基地,河南新乡453007 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南科学》2010年第3期296-300,共5页Henan Science

基　　金：国家973项目前期研究专项资助(2006CB708506)

摘　　要：为了解大鼠再生肝8种细胞的脂肪酸代谢基因转录谱及预示的生化活动,按张丽君[1]等方法分离大鼠再生肝8种细胞,检测它们的脂肪酸代谢基因表达变化,分析其表达相关性及预示的生理活动.结果表明,44个脂肪酸代谢基因在大鼠肝再生中发生了有意义表达变化,8种细胞的相应基因数为22、18、11、21、19、13、27、18,上调、下调和上/下调的基因个数为11、14和19,相应细胞的基因个数为6、15和1,7、8和3,3、8和0,16、3和2,12、7和0,8、5和0,6、21和0,9、5和4.肝细胞、胆管上皮细胞、星形细胞、库普弗细胞、陷窝细胞、树突状细胞等6种细胞的脂肪酸合成相关基因表达增强,肝细胞、星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞、树突状细胞等6种细胞的脂肪酸分解相关基因表达增强.上述结果预示大鼠肝再生中脂肪酸代谢活动增强,与大鼠肝再生密切相关.To explore the transcription profiles of fatty acid metabolism-related genes of rat 8 cell types of regenerating liver and the biochemical activities they predicted, the cells were isolated respectively, gene expression changes Zhang were detected, gene expression patterns and the predicted biochemical activities were analyzed following Lijun et al methods. The analysis suggested that 44 fatty acid metabolism-related genes taked place a meaningful expression changes during rat liver regeneration, the corresponding gene number in 8 liver cell types were 22, 18, 11,21,19, 13,27 and 8 respectively. The gene number of up- ,down- ,and up/down-regulated expression were 11, 14 and 19 respectively, the corresponding gene number in 8 liver cell types were 6, 15 and 1;7,8 and 3;3,8 and 0; 16,3 and 2; 12,7 and 0;6,21 and 0;8,5 and 0;9,5 and 4. It suggested that the fatty acid synthesis-related gene expression enhanced in hepatoeytes,bile duct epithelial ceils,hepatic stellate cells, Kupffer cells, pit cells and dendritic cells, and the degradation-related gene expression increased in hepatic stellate hepatocytes, cells,sinus endothelial cells,Kupffer cells,pit cells and dendritic cells. It predicts fatty acid metabolism strengthen in rat liver regeneration, and is closely associated with that.

关 键 词：大鼠肝再生 RAT GENOME 230 2.0芯片 基因转录谱 脂肪酸代谢 

分 类 号：Q418[生物学—生理学]