鼠尾藻多酚分级组分的抑菌活性研究  

Study on the antimicrobial activity of different fractions of phlorotannins from Sargassum thunbergii kuntze

作　　者：郭奇[1,2] 魏玉西[1] 殷邦忠[2] 刘淇[2] 刘蕾[1] 

机构地区：[1]青岛大学生物系,266071 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《渔业科学进展》2010年第1期117-121,共5页Progress in Fishery Sciences

基　　金：公益性行业(农业)专项(2009030300);青岛市科技计划项目(No.06-2212-JCH)共同资助

摘　　要：采用平板生长抑制法对鼠尾藻Sargassuzn thunbergii kuntze多酚化合物各分级组分进行抑菌活性研究。结果表明，在一定浓度范围内各分级组分对副溶血弧菌、哈维氏弧菌、沙蚕弧菌、金黄色葡萄球菌、大肠杆菌、鳗弧菌、溶藻弧菌和四联微球菌等受试菌株均有抑菌活性。其中，分级组分I（Mr〈5．0×10^3）的抑菌效果优于其他组分，对副溶血弧菌、哈维氏弧菌和沙蚕弧菌的最低抑菌浓度（MIC）值为900μg／ml，对金黄色葡萄球菌、大肠杆菌、鳗弧菌和溶藻弧菌的MIC值为1800μg／ml，对四联微球菌的MIC值为3600μg／ml。另外，组分Ⅰ（Mr〈5．0×10^3）显示出较好的热稳定性，在pH3～4时对受试菌的抑菌活性最强。The antimicrobial activity of different molecular-weight fractions of phlorotannins from Sargassurn thunbergii kuntze was screened by plate growth inhibition assay. The resuits indicated that all the fractions of phlorotannins can inhibit the growth of tested microorganisms including Vibrio parahaemolyticus, Vibrio harveyi, Vibrio nereis, Staphylococcus aureus , Escherichia coli , Vibrio anguillarum , Vibrio alginolyticus and Micrococcus tetragenus. Among them, the antibacterial activity of the lowest molecular-weight fraction I （Mr 〈 5.0×10^3） of phlorotannins was the best with the minimum inhibition concentration （MIC） values against the tested microorganisms above as 900,900,900,1 800,1 800,1 800,1 800 and 3 600 μg/ ml respectively. Additionally, phlorotannins from S. thunbergii kuntze showed excellent thermal stability, and the best antimicrobial activity to the tested bacteria was in pH 3-4.

关 键 词：鼠尾藻 褐藻多酚 抑菌活性 

分 类 号：Q949.206[生物学—植物学]