发酵工艺对重组人生长激素修饰变体的影响  

Effect of Fermentation Process on Recombinant Human Growth Hormone Modified Variants

作　　者：蒋靖欣[1] 王学东[1] 张建国[1] 

机构地区：[1]华东理工大学鲁华生物技术研究所,上海221008

出　　处：《重庆理工大学学报（自然科学）》2010年第2期29-33,共5页Journal of Chongqing University of Technology：Natural Science

基　　金：国家863高技术研究发展计划重点资助项目"生物技术药物中试放大及分离纯化技术平台"(2009ZX09306-001)

摘　　要：针对困扰产品纯度和活性的重组蛋白的稳定性问题,通过对发酵过程的控制,优化工艺保持细胞平稳生长。结果表明,优化后的工艺对rhgh的修饰突变影响显著,可以使rhgh突变体的含量降低到10%以下,降低了后续纯化工序的难度,提高了纯化收率。The stability of recombinant protein is a main issue that bothers the purity and activity of the products. By controlling and optimizing fermentation process, stable growth of cells is maintained. The results show that the optimized fermentation process has significant effect on rhgh mutations, can make the content of rhgh mutant reduced to 10% or less, and greatly reduces the difficulty of follow-up purification processes and therefore, improves the purification yield.

关 键 词：rhgh修饰突变体 发酵工艺 细胞代谢 

分 类 号：TQ131.21[化学工程—无机化工] Q575.11[生物学—生物化学]