肝内转移与多中心发生多结节肝癌的比较蛋白质组学分析  

Comparative proteomic analysis of differentiating multicentric occurrence and intrahepatic metastasis in multinodular hepatocellular carcinoma

作　　者：苏铭[1] 黎乐群[2] 彭涛[1] 郭雅[1] 肖开银[1] 尚丽明[1] 徐邦浩[1] 李仕来[1] 苏智雄[1] 叶新平[1] 

机构地区：[1]广西医科大学第一附属医院肝胆外科,广西南宁530021 [2]广西医科大学附属肿瘤医院肝胆外科,广西南宁530021

出　　处：《癌症》2010年第1期52-58,共7页Chinese Journal of Cancer

基　　金：国家自然科学基金项目(No.30460143;30560133);广西自然科学基金(桂科自0640101);广西地方性高发疾病重点实验室科学基金(桂科能0630006-5E1K);广西大型仪器协作共用网基金(No.468-2007-047);广西科学研究与技术开发计划项目基金(桂科攻0632007-1E)~~

摘　　要：背景与目的:多结节肝癌的起源既有单一原发肿瘤的肝内转移,又有各个结节单独存在的多中心性发生。我们对肝内转移组和多中心发生组多结节肝癌组织的全蛋白质表达谱进行比较分析,希望筛选出在多结节肝癌细胞克隆起源过程中发挥重要作用的差异蛋白分子。方法:应用双向凝胶电泳分离肝内转移组及多中心发生组多结节肝癌组织总蛋白,对两组间蛋白差异点应用质谱进行分析及数据库搜索、鉴定。结果:肝内转移组平均检测到(1025±52)个蛋白点(n=3),多中心发生组平均检测到(900±98)个蛋白点(n=3)。筛选出差异蛋白点25个,应用质谱鉴定出其中核纤层蛋白A/C异构体2等20种差异蛋白质。结论:多中心发生组与肝内转移组多结节肝癌的蛋白表达谱存在差异。鉴定出的20个差异表达蛋白可能参与肝癌的发生、发展、侵袭、转移,有可能成为潜在的、有应用价值的多结节肝癌细胞克隆起源鉴别分子标志。Background and Objective：Multinodular hepatocellular carcinoma （HCC） might originate from multicentric occurrence （MO） or intrahepatic metastasis （IM）.This study was to find out proteins which play important roles in clonal origin of multinodular hepatocellular carcinoma by screening the differentially expressed proteins between the MO and IM tissues using comparative proteomic analysis.Methods：Total protein extracted from the MO and IM tissues was separated by two-dimensional gel electrophoresis （2DE）.2-DE protein patterns between the MO and IM tissues were compared using computerized image analysis.Proteins exhibiting significant alternations were subsequently isolated and identified by mass spectrometry.Results：A total of （1025±52） and （900±98） spots could be detected in the protein profile in IM and MO tissues,respectively.The expression levels of 25 protein spots were statistically different between the two groups.Twenty of 25 protein spots were identified by MALDI-TOF-MS and bioi...更多nformatics.Conclusions：The protein profile of MO HCC tissues was different from that of IM HCC tissues.The twenty differentially expressed proteins might play key roles in the carcinogenesis and progression of multinodular HCC.These newly identified proteins might be potential and valuable biomarkers for identifying the clonal origin of multinodular HCC.

关 键 词：肝肿瘤 肝内转移 多中心发生 多结节性 蛋白质组 比较蛋白质组学分析 

分 类 号：R735.7[医药卫生—肿瘤]