sFlt-1基因转染多发性骨髓瘤细胞的体外实验研究  

In vitro study of human multiple myeloma cell transfected with soluble vascular endothelial growth factor receptor-1 gene

作　　者：郑冬[1] 李娟[1] 刘俊茹[1] 周振海[1] 谷景立[1] 

机构地区：[1]中山大学附属第一医院血液内科,广东广州510080

出　　处：《癌症》2010年第1期65-68,共4页Chinese Journal of Cancer

基　　金：广东省科技计划项目(No.2005B30301006)~~

摘　　要：背景与目的:血管内皮生长因子(vascular endothelial growth factor,VEGF)是多发性骨髓瘤(multiple myeloma,MM)最重要的促血管形成因子。本研究探讨人可溶性VEGF受体sFlt-1基因转染MM细胞株RPMI8226后对其生长的影响。方法:采用已构建的真核表达载体pcDNA3-sFlt-1,使用脂质体介导的方法转染RPMI8226细胞,RT-PCR及ELISA法检测sFlt-1的表达,MTT及ELISA法检测转染前后RPMI8226细胞生长及VEGF含量变化。结果:真核表达载体pcDNA3-sFlt-1成功转染RPMI8226细胞。转染后的RPMI8226细胞培养上清液中可检测到sFlt-1蛋白的表达,转染组RPMI8226细胞的生长受抑制,培养上清中VEGF的含量减少。结论:sFlt-1基因转染RPMI8226细胞后可表达具有生物活性的sFlt-1蛋白,抑制RPMI8226细胞的生长。Background and Objective：Vascular endothelial growth factor （VEGF） is the most important angiogenic factor of multiple myeloma （MM）.This study was to investigate the effect of transfection of human soluble vascular endothelial growth factor receptor-1 （sFlt-1） gene on the growth of human MM cell line RPMI8226.Methods：The sFlt-1 gene was obtained beforehand and preserved as recombinant plasmid pcDNA3-sFlt-1.The recombinant plasmid pcDNA3-sFlt-1 was transfected into RPMI8226 cells by lipofectamine 2000.The expression of sFlt-1 gene was identified by RT-PCR and ELISA.The effect of sFlt-1 protein on cell growth and VEGF expression in RPMI8226 cells was investigated by MTT and ELISA,respectively.Results：The recombinant plasmid pcDNA3-sFlt-1 was successfully transfected into RPMI8226 cells.And the sFlt-1 protein expression was identified by ELISA.The growth of RPMI8226 cells was inhibited and the VEGF level in the culture supernatant reduced.Conclusion：RPMI8226 cells can express sFlt-1 prote in with high biological activity when transfected by the sFlt-1 gene,which inhibits the growth of RPMI8226 cells.

关 键 词：血管内皮生长因子受体 转染 多发性骨髓瘤 RPMI8226细胞 

分 类 号：R733.3[医药卫生—肿瘤]