阿司匹林对萘性白内障α-晶状体蛋白分子伴侣活性的保护作用  

The protective effects of aspirin on the α-crystalline molecular chaperone-like activity in naphthalene-induced cataract

作　　者：陈燕[1] 卢奕[1] 蒋永祥[1] 邱斌[1] 田洁[1] 

机构地区：[1]复旦大学附属眼耳鼻喉医院眼科,上海200031

出　　处：《眼科研究》2010年第3期221-224,共4页Chinese Ophthalmic Research

摘　　要：目的探讨低剂量阿司匹林对萘性白内障α-晶状体蛋白分子伴侣活性的保护作用。方法将45只150～160g雌性SD大鼠随机分为空白对照组(无特殊处理)、萘性白内障组(每日0.5mg/kg萘灌胃3d后改为每日1mg/kg)和阿司匹林组(萘灌胃4h后每日100mg/kg阿司匹林灌胃)。应用定期图像分析和高效液相色谱(HPLC)法分离纯化α-晶状体蛋白,紫外分光光度法测定其抑制热诱导的β-L晶状体蛋白变性的能力。结果3周时萘性白内障组晶状体开始出现混浊,程度逐步加重,阿司匹林组混浊早期进展较萘性白内障慢,6周后进展程度接近于萘性白内障组。实验2周时3组晶状体混浊程度的比较差异无统计学意义(F=0.032,P=0.969)。实验第4、6、8、10周时3组晶状体混浊程度比较差异均有统计学意义(F=5031.130,P=0.000;F=115964.000,P=0.000;F=169846.500,P=0.000;F=195431.200,P=0.000),且空白对照组与萘性白内障组、空白对照组与阿司匹林组、阿司匹林组与萘性白内障组之间的差异均有统计学意义(P=0.000)。阿司匹林组的α-晶状体蛋白分子伴侣活性高于萘性白内障组。结论低剂量阿司匹林通过保护α-晶状体蛋白分子伴侣活性延缓萘性白内障大鼠晶状体混浊的进展,此作用在白内障早期尤为明显。Background Age-related cataract is the leading cause of visual impairment worldwide. To seek the effective prevention and drugs for management of cataract is important. Naphthalene-induced cataract of rat is an ideal animal model for the research of human age-related cataract, and aspirin has been proven to inhibit the development of human age-related cataract. Objective The present study is to investigate the role of aspirin on naphthalene-induced cataract. Methods Forty-five 150 - 160 g female SD rats were divided into three groups randomly. Naphthalene was orally taken with 0.5 mg/kg per day for 3 days and then 1 mg/kg per day for 70 days, and then 100 mg/kg of aspirin was given per day for 70 days following four-day washout period in group A. In group B,the animals was given orally only naphthalene at the same way. No any intervention was used in group C. Naphthalene-induced cataract was examined under the slim lamp every week. The experimental animals were sacrificed and lenses were obtained in 70 days. α-Crystalline was extracted from lens homogenate and purified and identified using High Performance Liquid Chromatography （HPLC）, 2-dimentional electrophoresis gel and Western blot. Different abilities of a-crystalline to protect β low crystalline from aggregation were observed using ultraviolet spectrophotometer. Results Naphthalene-induced cataract formed at the third week in only naphthalene group but at the sixth week in naphthalene ＋ aspirin group under the slim lamp. No significant difference was found in the degree of lenses opacity in the second week among these three groups（ F = 0. 032 ,P = 0. 969）. Howevcr,a statistically significant difference was seen in the degree of lenses opacity in the fourth, sixth, eighth and tenth week among these three groups（F = 5 031. 130,P =0.000;F = 115 964.000,P =0.000;F = 169 846. 500,P = 0. 000;F = 195 431. 200, P = 0. 000）. The molecular chaperone-like activity was significantly higher than that of the naphthalene-induced group. Conclusion Aspirin d

关 键 词：萘性白内障 阿司匹林 Α-晶状体蛋白 分子伴侣活性 

分 类 号：R776[医药卫生—眼科]