牵张成骨矫治猕猴腭部软硬组织缺损的组织学及荧光标记研究  

Distraction osteogenesis for correction of cleft palate in rhesus-histological and fluorescent labeling study

作　　者：刘毅[1] 陈刚[1] 刘延山[1] 申岱[1] 朱彤[1] 王之奇[1] 

机构地区：[1]天津医科大学口腔医院口腔颌面外科,天津300070

出　　处：《中华整形外科杂志》2010年第1期43-47,共5页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助项目（30500533）

摘　　要：目的观察猕猴牵张成骨术整复腭裂过程中，新骨组织形成与改建活动的特点，探讨新骨生成的规律。方法建立猕猴腭裂动物模型，以牵张成骨术整复其腭部软硬组织缺损，以每天2次、每次0．4mm的速率牵引，直至骨运送盘移动关闭裂隙后原位固定。分别于固定期第1、2、4、6、8、12及24周取材，每时相点3只实验动物。取材前6d，实验组动物（21只）均肌肉注射四环素（30mg／kg）标记。各组标本包埋切片行组织学及荧光标记观察。结果与实验对照组及空白对照组（动物各2只，同法标记）对比。结果实验组骨运送盘移动至裂隙对侧关闭缺损，牵开间隙内以膜内成骨方式原位成骨，成功整复腭部软硬组织缺损。牵张成骨术后固定期从1至24周逐渐延长，呈现出成骨-改建-成熟的动态变化过程：牵张区新骨形成的量及其钙化程度不断增加，与此同时，新骨组织亦呈现不断改建成熟的趋势，软组织随着新骨的形成不断地延伸，对照组裂隙无法自行修复。结论应用牵张成骨术整复腭裂缺损，在良好的固定下，通过膜内成骨的方式，新骨不断的成熟和改建，直至成功修复软硬组织缺损。Objective To investigate the mechanism of new bone formation in the distraction osteogenesis （DO） for correction of cleft palate（EP） in rhesus. Methods CP was created by operation in 23 rhesus. The CP was corrected with DO in 21 animals as experimental group. The distraction rate was 0. 8mm per day, two times a day. The bone fragments were fixed after cleft closure, every 3 animals were sacrified to get specimen after 1, 2, 4, 6, 8, 12, 24 weeks of fixation. 6 days before sacrifice, tetracycline was administrated for labeling （30 mg/kg）. Results The hard and soft tissue def of fixation. At the same time, the bone volume and calcification between the distraction gap increased. The cleft in the control group could not b ect was successfully closed with DO by intramembrane osteogenesis. The new formed bone was remodeling and became maturation during the period e corrected spontaneously. Conclusions The DO can successfully correct both the soft and hard tissue defect in CP by intramembrane osteogenesis. The fixation is important for remodeling and maturation of the new formed bone.

关 键 词：骨生成 牵张 腭裂 组织学观察 荧光标记 

分 类 号：R782.2[医药卫生—口腔医学]