NS398逆转溶血磷脂酸拮抗顺铂抑制卵巢癌细胞增殖作用的研究  

Study of NS398 reversing the antergy of lysophosphatidic acid for the inhibiting effect of cisplatin in the proliferation of huanman ovarian cancer

作　　者：熊学华[1] 彭真年[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016

出　　处：《重庆医科大学学报》2010年第2期209-212,共4页Journal of Chongqing Medical University

摘　　要：目的:观察环氧化酶-2(Cyclooxygenase-2,COX-2)抑制剂NS398对溶血磷脂酸(Lysophosphatidic acid,LPA)拮抗顺铂抑制卵巢癌细胞细胞增殖作用的影响,并探讨其可能机制。方法:体外培养人卵巢癌细胞3AO,MTT法检测NS398对LPA拮抗顺铂抑制3AO细胞增殖的影响;流式细胞仪检测细胞周期变化;RT-PCR检测LPA单独或合用NS398对3AO细胞表达COX-2的影响。结果:LPA对顺铂抑制卵巢癌细胞增殖有拮抗作用,并降低G_0/G_1期的细胞比率;联合用NS398后,卵巢癌细胞的生长显著受抑制,同时G_0/G_1期细胞比率上升;RT-PCR结果显示40μmol/L LPA能促进COX-2的表达,而合用NS398后COX-2表达降低。结论:NS398可能通过抑制COX-2表达,实现逆转LPA拮抗顺铂抑制卵巢癌细胞细胞增殖的作用。Objective： To investigate the effect of NS398,cyclooxygenase-2（COX-2）-inhibitor,on the antergy of lysophosphatidic acid （LPA） for the inhibiting effect of cisplatin（DDP） in the proliferation of huanman ovarian cancer , and to analyze its possible mechanism. Methods： Human ovarian cancer cell line（ 3AO ） were cultured in vitro. The effect of NS398 on the antergy of LPA for the inhibiting effect of DDP in the proliferation of 3AO cells were measured by MTT. Cell cycle of 3AO were analysed by flow cytometry （FCM）. The effect of LPA alone or in combination with NS398 on the expression of COX-2 of 3AO cells were measured with RT-PCR. Results ： LPA showed antergy on the inhibiting effect of DDP in the proliferation of 3AO cells, and decrease the proportion of cells in the G0/G1 phase of the cell cycle. Whereas, combined with NS398 in culture, the proliferation of were significantly inhibited, and the the proportion of cells in the G0/G1 phase of the cell cycle was raised.RT-PCR results revealed that 40 μmol/L LPA could promote the expression of COX-2 in 3AO cells,and in combination with NS398,the expression of COX-2 was decreased.Conclusion： The reverse effect of NS398 for the antergy of LPA on the inhibiting effect of DDP in the proliferation of 3AO cells may be completed by inhibiting the expression of COX-2 in these cells.

关 键 词：溶血磷脂酸 环氧化酶-2 顺铂 NS398 3AO细胞 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]