甲胎蛋白异质体和甲胎蛋白信使核糖核酸用于诊断肝细胞癌  

Application of Combined Detection of AFP-L3 and AFP mRNA in the Diagnosis of Hepatocellular Carcinoma

作　　者：韩风[1] 邱大鹏[1] 陈伟瑜[1] 张玲[1] 韩有志[1] 

机构地区：[1]河南省肿瘤医院肝胆胰外科,河南郑州450003

出　　处：《中国临床医学》2010年第1期45-46,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:探讨联合检测血清肿瘤标志物甲胎蛋白(AFP)异质体(AFP-L3)和甲胎蛋白信使核糖核酸(AFPmRNA)诊断肝细胞癌及评估肝癌预后的价值。方法:对2005年4月—2005年7月收治的82例肝细胞癌(HCC)、11例慢性乙型肝炎肝硬化合并门脉高压和19例肝脏良性肿瘤患者,应用酶联吸附法及荧光定量反转录-聚合酶链反应(RT-PCR)联合检测AFP-L3和AFPmRNA水平,对比其阳性率。结果:AFP-L3和AFPmRNA联合检测阳性率可达87.6%,较单独检测AFP、AFP-L3、AFP-mRNA的阳性率(分别为69.5%、81.7%、36.6%)高,差异有统计学意义;术前、术后联合检测AFP-L3和AFPmRNA均阳性患者的术后3年生存率较低。结论:术前联合检测APF-L3和AFPmRNA可提高HCC,尤其是AFP阴性HCC的诊断率,而术后联合检测对评估HCC的预后有一定的指导意义。Objective：To invesigate the value of detecting alpha fetoprotein variant （Lens culinaris agglutinin-reactive alpha-fetoprotein,AFP L3） and alpha-fetoprotein messenger RNA（AFPmRNA） in the diagnosis and prognosis of hepatocellular-carci- noma （HCC）. Methods：Serum alpha fetopro tein variant and AFPmRNA were measured in 82 cases with HCC, 11 cases with chronic hepatitis and 19 cases with benign tumors, all cases were accepted in our hospital from April to July, 2005. Then their positive rates were calculated and compared. The level of alpha fetoprotein variant and AFPmRNA were also measured in 82 cases with HCC after operation. Results： The combined positive rate of AFP-L3 and AFPmRNA was 87.6%, which was higher than single positive rate of AFP,AFP L3 and AFPmRNA were 69.50//00 , 81.70~ and 36.60//oo , respectively. The combined posi- tive rate has statistical significance compared with that of AFP or AFPmRNA. Furthermore,combined detection of serum tumor markers was valuable in the prognosis of HCC. Conclusions.. The combined detection of tumor markers like alpha-fetoprotein variant（AFP- L3） and alph^fetoprotein messenger RNA（AFPmRNA） is valuable in the diagnosis and prognosis of HCC.

关 键 词：原发性肝癌 甲胎蛋白异质体 甲胎蛋白信使核糖核酸 诊断 预后 

分 类 号：R735.7[医药卫生—肿瘤]