β-榄香烯乳联合照射对肺腺癌A549细胞凋亡及KU70基因表达的影响  

Effect of β-elemene Combined with Radiotherapy on the Expression of KU70 mRNA and Apoptosis of Lung Adenocarcinoma Cell Line A549

作　　者：张卓[1] 邹丽娟[1] 田莹莹[2] 

机构地区：[1]大连医科大学附属第二医院肿瘤放疗科,辽宁省大连市116023 [2]青岛市肿瘤医院肿瘤科

出　　处：《中国肿瘤临床》2010年第4期184-186,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30770646)~~

摘　　要：目的:检测β-榄香烯乳对肺腺癌A549细胞株放射增敏作用,探讨β-榄香烯乳影响细胞凋亡及Ku70基因mRNA表达的放射增敏机制。方法:四氮唑蓝比色分析法(MTT法)检测β-榄香烯乳对A549细胞的半数抑制浓度(IC50);将细胞分为对照组(C)、照射组(IR)、β-榄香烯乳组(0.1×IC50、0.2×IC50)及β-榄香烯乳联合照射组(0.1×IC50+IR、0.2×IC50+IR),不同浓度的β-榄香烯乳处理细胞24h后照射,通过克隆形成实验计算细胞存活率;流式细胞仪检测细胞周期与凋亡率;逆转录PCR技术(RT-PCR)检测细胞KU70基因mRNA表达量。结果:MTT法测得β-榄香烯乳对A549细胞的IC50值为120μg/mL;克隆形成实验证明β-榄香烯乳对A549细胞有放射增敏作用;β-榄香烯乳联合照射组细胞C_2/M期比率及凋亡率明显高于单纯照射组及β-榄香烯乳组(P<0.05);β-榄香烯乳联合照射组细胞KU70基因mRNA表达量较照射组明显下降(P<0.05)。结论:β-榄香烯乳可促进A549细胞凋亡和抑制KU70基因mRNA表达,诱导凋亡同时抑制KU70参与的双链损伤修复途径可能是其放射增敏的机制。Objective： To study the effect of β-elemene combined with radiotherapy on the expression of KU70 mRNA and apoptosis of lung adenocarcinoma cell line A549. Methods： A549 cells were divided into the control group （c）, irridation group （IR）, β-elemene group （0.1 × IC50 and 0.2 × IC50 i.e. 10 μg/mL and 20μg/mL β-elemene ） and β-elemene combined with irridation group （0.1 × IC50 ＋ IR and 0.2 × IC50 ＋ IR）. IC50 was obtained through MTT method and cell survival rate was analyzed by colony formation test. Cell cycle and apoptosis were detected with flow cytometry. The expression level of KU70 mRNA was detected by RT-PCR. Results： MTT assay showed that IC50 value of A549 cells was 120 μg/mL. The number of cell clones and survival rate of β-elemene groups were declined significantly. The radiosensitivity of A549 cells can be enhanced byβ-elemene. The flow cytometry confirmed that the ratio of GJM and apoptosis were significantly increased under the effect of 13-elemene, statistically different from those in the control group （P 〈0.05）. The expression level of KU70 mRNA in β-elemene with radiotherapy group was declined significantly, statistically different from those in the control group （P 〈0.05）. Conclusion： The radiosensitization effect of β-elemene on A549 cells is associated with induction of cell apoptosis and down-regulation of KU70 mRNA expression.

关 键 词：β-榄香烯乳 KU70 凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R734.202[医药卫生—临床医学]