促凋亡基因PDCD5在新疆哈萨克族与汉族食管癌中的表达及临床意义  

Apoptosis-associated Gene PDCD5 Expression in Esophageal Cancer in Xinjiang Kazakh and Han Nationality

作　　者：郭辉[1] 丁剑冰[1] 孙伟[2] 马秀敏[1] 张彤[1] 曹春宝[1] 陈英玉[3] 

机构地区：[1]新疆医科大学基础医学院免疫学教研室,乌鲁木齐市830011 [2]新疆医科大学附属肿瘤医院胸外科 [3]北京大学人类疾病基因研究中心

出　　处：《中国肿瘤临床》2010年第4期213-215,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30871263;30771057);新疆医科大学科研创新基金资助(编号:20082308)~~

摘　　要：目的:探讨新疆哈萨克族与汉族食管鳞癌组织中PDCD5 mRNA的表达及其临床意义。方法:以PDCD5 mRNA为靶基因运用RT-PCR法检测40例食管鳞癌患者中PDCD5 mRNA的表达(哈萨克族18例、汉族22例)。结果:40例食管鳞癌患者癌组织、癌旁组织、正常组织中PDCD5 mRNA表达阳性率为80.0%、80.0%、87.5%,差异均无统计学意义(P>0.05)。半定量光密度计数后PDCD5 mRNA表达量在食管鳞癌患者癌组织、癌旁组织、正常组织中分别为0.7644±0.1444、0.9341±0.1631和1.8703±0.4767,其表达在癌组织与正常组织中差异有统计学意义(P<0.05)。PDCD5 mRNA的表达与分化程度有无淋巴结转移均无相关性。结论:PDCD5 mRNA的表达在不同民族之间无显著性差异。PDCD5 mRNA的表达与食管癌的发生、发展、远处转移和细胞分化程度均无密切相关性,可以通过增加例数对其关联性进行深入研究;PDCD5 mRNA在食管癌患者癌组织中的表达量低于正常组织,本研究可能对食管癌的治疗提供理论依据。Objective： To study the expression of PDCD5 mRNA and its significance in esophageal cancer in Xinjiang Kazakh and Han nationality. Methods： RT-PCR was used to detect the mRNA of PDCD5 in 40 cases of esophageal cancer （18 cases of Kazakh, 22 cases of Han）. Results： The positive rate of PDCD5 mRNA expression in 40 samples of esophageal cancer tissue, adjacent tissue, and normal tissue was 80.0% （32/40）, 80.0% （32/40） and 87.5% （35/40）, respectively, with no significant difference （P〉0.05）. The ODs of cancer tissues, adjacent tissues, and normal tissues were 0.7644± 0.1444, 0.9341±0.1631 and 1.8703±0.4767, respectively. The expression of PDCD5 was significantly increased in cancer tissues compared with that in normal tissues （P〈0.05）.The expression level of PDCD5 mRNA was not significantly correlated with the degree of differentiation and lymph node metastasis. Conclusion： No significant difference was found in PDCD5 mRNA expression in esophageal cancer between Xinjiang kazakh and Han nationality （P〉0.05）. The expression of PDCD5 is not correlated with the degree of differentiation, depth of invasion or lymph node metastasis. Detection of PDCD5 mRNA expression in esophageal cancer tissues may provide valuable information for patient prognosis.

关 键 词：食管癌 程序化细胞死亡分子5 逆转录-聚合酶链式反应 民族 

分 类 号：R735.1[医药卫生—肿瘤]