针灸治疗帕金森病可能氧化应激作用机制的研究  

The possible mechanism of oxidative stress on acupuncture treatment in the rat of parkinson's disease

作　　者：袁红[1] 刘平[2] 郑春秀[1] 王向党[1] 张振文[1] 李春雷[1] 焦淑军[1] 白丽敏[3] 梁立武[1] 

机构地区：[1]武警总医院中西医结合科,北京100039 [2]哈尔滨医科大学免疫教研室 [3]北京中医药大学中西医结合基础解剖教研室

出　　处：《中华保健医学杂志》2010年第1期45-47,共3页Chinese Journal of Health Care and Medicine

基　　金：第41批中国博士后资助项目(20070410569);黑龙江省自然科学基金资助项目(D2007-63)

摘　　要：目的探讨针灸对治疗帕金森病(PD)可能的氧化应激作用机制。方法在脑定位仪下,将6-羟基多巴注入左侧纹状体造模,连续2周针灸治疗,1/d。2周后,测定血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量。结果与正常组比较,大鼠PD造模成功后,SOD活力升高(104.33±7.91U/μgvs112.85±3.33U/μg,P<0.05),GSH-PX活力降低(709.65±29.86U/μg-1·min-1vs686.94±21.94U/μg-1·min-1,P<0.05),MDA含量升高(22.93±1.85nmol/mgvs28.74±0.79nmol/mg,P<0.05)。与模型组比较,针灸组、美多巴+针灸组均能提高SOD活力分别为130.14±5.08、124.55±12.40U/μg,(P<0.01),提高GSH-PX活力分别为777.55±39.36、700.51±18.93U/μg-1·min-1,(P<0.01,P<0.05),降低MDA含量分别为15.35±1.68、18.03±3.31nmol/mg,(P<0.01),且针灸组作用显著。结论在6-羟基多巴毁损纹状体模型中,针灸治疗显著提高SOD活力和GSH-PX活力,降低MDA含量,针灸可能参与PD氧化应激机制的调节。Objective We investigated the possible neuroprotective effect of acupuncture on the mechanism of oxidative stress in the rat of Parkinson＇s disease.Methods The rats received a unilateral 6-hydroxydopamine（6-OHDA）injection into the left striatum.Acupuncture intervention was performed within 2 weeks by post-lesioning.The expression of SOD、GSH-PX and MDA was evaluated by chemistry luminesce method、DTNB colorimetry and TBA reaction method.Results Compared with normal group,the effect of SOD is increased in model group（104.33±7.91 U/μg vs 112.85.±3.33 U/μg,P0.05）,the effect of GSH-PX reduced（709.65±29.86 U/μg-1·min-1 vs 686.94.±21.94 U/μg-1·min-1,P0.05）,The content of MDA increased（22.93±1.85 nmol/mg vs 28.74.±0.79 nmol/mg,P0.05）.Compared with model group,acupuncture group Madopa/acupuncture group can increase the effect of SOD（130.14±5.08、124.55±12.40 U/μg,P0.01）and GSH-PX（777.55±39.36、700.51±18.93 U/μg-1·min-1,P0.01,P 0.05）,decrease the content of MDA（15.35±1.68、18.03±3.31 nmol/mg,P0.01）,respectively.Acupuncture-treatment,significantly raise the effect of SOD、GSH-PX,attenuated the expression of MDA.Conclusions In 6-OHDA lesion striatum model,acupuncture-treatment significantly elevated the effect of SOD GSH-px,attenuated the expression of MDA.Acupuncture may take part in adjust of mechanism of oxidative stress in Parkinson＇s disease.

关 键 词：帕金森病 针灸 6-羟基多巴 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：R742.5[医药卫生—神经病学与精神病学]