幽门螺杆菌cagA和hrgA基因与胃癌慢性胃炎相关性研究  

Correlative Study on CagA and HrgA Genes of H Pylori in Gastric Carcinoma and Atrophic Gastritis

作　　者：王景毅[1] 韩梅娇[1] 罗荣[1] 

机构地区：[1]黑龙江省医院普外科,黑龙江省哈尔滨150001

出　　处：《中国基层医药》2010年第3期293-294,共2页Chinese Journal of Primary Medicine and Pharmacy

基　　金：黑龙江省卫生厅基金资助项目（2003137）

摘　　要：目的探讨幽门螺杆菌（Hp）菌株中cagA和hrgA基因对胃癌、慢性萎缩性胃炎的致病作用及其检测的意义。方法胃镜下诊断慢性胃炎的病例，钳取活体组织标本，组织学检查。胃癌及消化性溃疡术后切除标本，组织学检查确认，快速尿素酶法和聚合酶链反应（PCR）检测。结果胃腺癌中18例hrgA阳性、6例hrgA阴性，20例cagA阳性、4例cagA阴性；消化性溃疡中6例hrgA阳性、8例hrgA阴性，12例cagA阳性、2例cagA阴性；慢性萎缩性胃炎中30例Hp阳性、8例Hp阴性，22例cagA阳性、16例cagA阴性；慢性浅表性胃炎中2例cagA阳性、20例cagA阴性。结论Hp感染与胃癌、慢性萎缩性胃炎的发生有密切关系。PCR检测较快速尿素酶法准确。检测cagA和hrgA基因对了解Hp菌株的致病性、估计疾病程度、了解病变预后及临床治疗都具有重要意义。Objective To investigate the role of hrgA and cagA genes of H. Pylori causing diseases and the value of detection in gastric carcinoma and atrophic gastritis. Methods Gastric biopsy specimens from 26 patients with gastric carcinoma and 14 with pepticulcer were obtained, and were performed histopathological examination, rapid urease test and polymerase chain reaction （PCR）. Results Among the detected 26 gastric carcinoma cases,24 cases were gastric adenoearcinoma and 2 eases were gastric MALT lymphoma. Hp was present in 12 of 24 cases with gastric adenocareinoma and in 12 of 14 cases with peptic ulcer by rapid urease test. Of the 24 cases with gastric adenoearcinoma, 18 were hrgA positive and 20 were eagA positive;of the 14 eases with peptieulcer,6 were hrgA positive and 12 was eagA positive by PCR. Conclusions Hp could cause gastric carcinoma,and PCR was more reliable than the urease methods. To detect hrgA and cagA of Hp was very important for realizing the role of causing disease, assessing the damage, previewing the disease and choosing clinical treatment.

关 键 词：胃肿瘤 胃炎 萎缩性 限制性内切酶置换基因 螺杆菌 幽门 

分 类 号：R735[医药卫生—肿瘤] R573[医药卫生—临床医学]