响应面法优化固定化碱性蛋白酶工艺  

OPTIMIZATION OF ALKALINE PROTEASE IMMOBILIZATION BY RESPONSE SURFACE METHODOLOGY

作　　者：侯利霞[1] 相朝清[1] 王金水[1] 赵舒畅[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450052

出　　处：《河南工业大学学报（自然科学版）》2010年第1期53-58,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南工业大学引进人才项目(150288);郑州市科技攻关计划项目(083SGYG25121-6)

摘　　要：采用多孔壳聚糖微球吸附和戊二醛交联的方法,将碱性蛋白酶固定于壳聚糖上.在pH、加酶量、温度、戊二醛体积分数和固定化时间5个单因素试验的基础上,利用响应面分析法对固定化碱性蛋白酶的工艺条件进行了优化.结果表明:在pH 9.20、加酶量7 512 U/g、固定化温度44.58℃、戊二醛体积分数0.20%、固定化时间8.11 h的最佳工艺条件下,酶的回收率高达56.73%.Alkaline protease Alcalase 2.4L was immobilized on chitosan by using porous chitosan microsphere adsorption and glutaraldehyde crosslinking method. Based on five single - factor tests concerning pH, protease amount, temperature, glutaraldebyde volume fraction and time, the process conditions for the immobilization of alkaline protease were optimized by response surface methodology. The results show that the recovery rate of protease is up to 56.73% under the optimum conditions of pH 9.20, protease amount 7 512 U/g, immobiliza- tion temperature 44.58 ℃ , glutaraldehyde volume fraction 0.20% and immobilization time 8.11 hours.

关 键 词：固定化 碱性蛋白酶 响应面 优化 

分 类 号：TS201.2[轻工技术与工程—食品科学]