蜈蚣提取液对裸鼠移植肝癌抑癌作用及机制的研究  

Study on the mechanisms of tumor inhibitory effect of centipede extraction on heterotopic grafting hepatocellular carcinoma in nude mice

作　　者：刘细平[1] 钟德玝[2] 

机构地区：[1]湖南省株洲市一医院普通外科,湖南株洲412000 [2]中南大学湘雅二医院普通外科,湖南长沙410008

出　　处：《中国普通外科杂志》2010年第2期164-168,共5页China Journal of General Surgery

摘　　要：目的探讨蜈蚣提取液对裸鼠移植肝癌的治疗作用及机制。方法建立裸鼠Bel-7404人异位肝癌移植模型,以蜈蚣提取液灌胃31d后,用免疫组化法对肿瘤组织标本进行X染色体连锁凋亡抑制蛋白(XIAP)、Bax基因、血管内皮细胞生长因子(VEGF)和促血管生成素2(Ang-2)的检测。结果对照组XIAP,VEGF与Ang-2的染色细胞数、染色强度及OD值均较治疗组多而强,而Bax的染色强度、染色细胞数及OD值较治疗组少而弱,差异均有统计学意义(P<0.01)。结论蜈蚣提取液能抑制裸鼠Bel-7404移植瘤的生长,其机制与抑制肿瘤血管生成、促进肿瘤细胞凋亡有关。Objective To investigate the effects and mechanisms of extraction centipede extraction （ CE ） on grafting hepatocellular carcinoma （ HCC ） in nude mice. Methods We set up model of Bel-7404 cells heterotopic grafting HCC in nude mice, and observed the tumor growth and metastasis after intragastric administration of CE （treatment group ）. Immunohistochemical methods were used to detect X-chromosome linked inhibitor of apoptosis protein （ XIAP ） , bax gene, vascular endothelial growth factor （ VEGF ） and angiopoietin-2 （Ang-2） in tumor tissue. Results There was distinctive inhibitive effect of CE on in Bel-7404 ceils heterotopic grafting HCC in nude mice. The staining cells population, staining intensity and the optical density （OD） of XIAP, VEGF and Ang-2 in the control group were higher than those in treatment group, but those of BAX were lower than that in treatment group, and there was significant difference between the 2 groups （ P 〈 0. 05 ）. Conclusions CE can inhibit the growth of heterotopic grafting HCC in nude mice, and the mechanisms may be related to inhibition of tumor angiogenesis and promotion of apoptosis of HCC.

关 键 词：癌 肝细胞 蜈蚣提取液 移植 异种 模型 疾病 

分 类 号：R735.7[医药卫生—肿瘤]