产高温碱性蛋白酶菌株筛选及酶学性质研究  

Screening and Characterization of an Thermophilic Alkaline Crude Protease from an Isolated Strain of Bacillus subtilis I15

作　　者：翁海波[1] 敬蔚然[1] 杨丹丽[1] 柳丽平[1] 王志强[1] 

机构地区：[1]郑州大学生物工程系,郑州河南450001

出　　处：《郑州大学学报（工学版）》2010年第1期70-73,共4页Journal of Zhengzhou University（Engineering Science）

基　　金：河南省重点科技攻关项目(072102220002)

摘　　要：从高温堆肥土样中筛选到一株高产蛋白酶的菌株,经鉴定为Bacillus subtilis,命名为I15.对最适产酶发酵条件进行了优化,并研究了发酵产蛋白酶的酶学性质.结果表明,玉米淀粉和豆饼粉是该菌发酵产蛋白酶的最适经济碳氮源;该蛋白酶属高温碱性蛋白酶,具有很强的热稳定性和酸碱稳定性.另外,突出的性质是该蛋白酶对表面活性剂和温和型去垢剂具有超强的耐受性,将具有广阔的市场前景.A strain producing higher activity protease named Bacillus subtilis I15 is isolated and identified from waste-rich soil which stacked high temperature and long time.We optimize the fermentation conditions of this strain and study a part of enzymology properties.The results show that corn starch and soybean cake powder are the optimization of this strain as carbon and nitrogen source for thermophilic alkaline protease production.The protease belongs to thermophilic alkaline protease,which shows high pH stability and thermal stability.In addition,it shows excellent stability and compatibility with surfactant and detergent.These properties indicate that the protease has a bright market prospect.

关 键 词：高温碱性蛋白酶 筛选 酶学性质 

分 类 号：Q55[生物学—生物化学]