烟草环斑病毒PCR产物的克隆及部分序列分析  

The cloning and partial sequencing of Tobacco ringspot virus PCR product.

作　　者：相宁[1,2,3] 孙彤[1,2,3] 刘宏迪 张成良[1,2,3] 杨立[1,2,3] 

机构地区：[1]农业部植物检疫实验所 [2]中科院微生物所 [3]中国农业大学

出　　处：《植物检疫》1998年第5期260-263,共4页Plant Quarantine

摘　　要：将ＴＲＳＶ的ＰＣＲ产物与ｐＧＥＴ－ＴＥａｓｙＶｅｃｔｏｒ连接，克隆到大肠杆菌ＴＧ１中，得到白色菌落，重组质粒通过酶切鉴定、ＰＣＲ扩增和部分序列分析，表明ＴＲＳＶ的ＰＣＲ产物确实插入了质粒，并已克隆到大肠杆菌中，测序列２４０个碱基，与资料显示的序列相比较，同源性达９２．５％，可用于解决病毒检疫应用中阳性对照有扩散危险的疑难问题。TRSV PCR product was cloned into pGEM T Vector and E.coli TG1 was transformated. Some white colonies were systematically studied. The results of restriction enzyme digestion, PCR amplification and partial sequencing showed that TRSV PCR product was inserted into plasmid and transformated E.coli TG1. The nucleotide sequence of 240bp was obtained and compared with the published TRSV sequence, showing 92.5% homology. The cloned PCR products can be used as positive control and eliminate the risk of TRSV spread by intact virus.

关 键 词：烟草 环斑病毒 PCR产物 序列分析 克隆 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]