绿茶提取物对香烟烟雾致人支气管上皮细胞氧化损伤的干预作用  

Intervention of Green Tea Extract on Oxidative Stress Caused by Cigarette Smoke in Human Bronchial Epithelial Cells in vitro

作　　者：叶飞[1] 潘宇[1] 蔡淑娴[1] 刘仲华[1] 黄建安[1] 

机构地区：[1]湖南农业大学茶学教育部重点实验室,长沙410128

出　　处：《天然产物研究与开发》2009年第B05期216-219,共4页Natural Product Research and Development

基　　金：基金项目：湖南省重大科技专项（2008FJ1005）;科技部支撑计划重点项目（2007BAD58B00-03）

摘　　要：以烟草烟雾水溶液（CSS）为材料，用MTT等方法研究CSS对人支气管上皮细胞（HBE细胞）的损伤及绿茶提取物对HBE细胞的保护作用及机理。结果表明：CSS抑制了HBE细胞活性，并呈良好的剂量关系；绿茶提取物在低浓度时（0～100μg／mL）对CSS引起的HBE氧化损伤有较好的保护作用，但在高浓度下（100～150μg／mL）促氧化作用将急剧上升，反而加剧了CSS所引起的氧化损伤。This study was conducted to identify the protection of Green tea extract to the human bronchial epithelial cells damage by oxidant-stress. Cigarette smoke solution（CSS） were used to induce the renal damage of HBE cells in vitro. With the model of cell oxidant-stress, the inhibition effects of CSS on the growth of HBE cells were investigated by MTT assay. The results showed that CSS decreased the viability of HBE cells in a dose-dependent manner. Green tea extract significantly protect the viability of HBE cells induced by CSS in low concentration （0-100μg/mL）, but the oxidative stress will be promoted in high dose of Green tea extract（ 100-150 μg/mL） for human bronchial epithelial cells.

关 键 词：人支气管上皮细胞 烟草烟雾水溶液 乳酸脱氢酶 丙二醛 活性氧 绿茶提取物 

分 类 号：Q946.91[生物学—植物学] R285[医药卫生—中药学]