胶束溶液中微生物萃取转化合成L-苯基乙酰基甲醇条件的研究  

Study on conditions of extractive microbial transformation to produce L-phenylacetylcarbinol in micelle aqueous solution

作　　者：薛迎迎[1,2] 杨汝德[1] 齐瀚实[2] 王志龙[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510006 [2]上海交通大学药学院,上海200240

出　　处：《食品科技》2010年第2期2-5,共4页Food Science and Technology

基　　金：国家自然科学基金项目(20676080)

摘　　要：对非离子表面活性剂胶束溶液中微生物萃取转化合成L-PAC的条件进行了优化研究,包括pH、TritonX-100、底物苯甲醛、湿酵母细胞量、葡萄糖、氮源和转化时间。最后确定最佳的转化培养基配方为:底物苯甲醛0.9%,TritonX-1007.8%,葡萄糖3%,湿酵母细胞量12%,pH5.2,转化时间为6h,不添加任何氮源。在此条件下,产物L-PAC的产量为45mmol/L(约7g/L)。This paper researched the conditions of extractive microbial transformation to produce L-PAC in nonionic surfactant micelle aqueous solution, including pH, Triton X-100, substrate benzaldehyde, wet cell dose, glucose, nitrogen source and transformation time. The optimized transformation medium was as follows： substrate concentration 0.9%, Triton X-100 7.8%, glucose 3%, wet cell dose 12%, pH5.2 and transformation time 6 h without any nitrogen source. Under the above optimum condition, a high product L-PAC concentration of 45 mmol/L, about 7 g/L was achieved.

关 键 词：非离子表面活性剂 胶束 微生物萃取转化 L-苯基乙酰基甲醇 

分 类 号：TS201.3[轻工技术与工程—食品科学]