NK细胞杀瘤活性与肿瘤细胞表面MICA/B表达的相关性研究  

The Relationship between the MICA/B Expression on Cancer Cell Lines and Their Sensitivity to NK Cell Cytotoxicity

作　　者：王新利[1] 李卿[1] 蒋涛[1] 王扬[1] 付立叶[1] 高婷[1] 姜又红[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第二研究室,沈阳110001

出　　处：《中国医科大学学报》2010年第2期95-97,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20042093)

摘　　要：目的探讨自然杀伤NK细胞杀瘤活性与肿瘤细胞表面MICA/B表达的相关性。方法采用流式细胞术检测人红白血病细胞K562、乳腺癌细胞Bcap37、肾癌细胞769P及肺癌细胞A549表面MICA/B的表达,RosetteSep誖改进法分离人外周血NK细胞,MTT法检测不同效靶比时NK细胞对几种肿瘤细胞的杀伤活性。结果K562、Bcap37、769P及A549细胞表面MICA/B的表达阳性率分别是(60.35±0.50)%、(90.72±0.64)%、(55.59±0.55)%、(3.84±0.10)%;RosetteSep誖改进法分选出的NK细胞纯度为(96.52±2.42)%,在同一效靶比时,NK细胞对K562、Bcap37及769P的杀伤活性较强,与A549相比有显著性差异(P<0.01),其中K562与Bcap37之间无显著性差异(P>0.05);效靶比为5∶1、10∶1、20∶1时,NK细胞的杀伤活性与4种肿瘤细胞表面MICA/B表达均呈正相关关系(P<0.01),随着效靶比增加,相关性增强。结论NK细胞的杀伤敏感性与肿瘤细胞表面MICA/B的表达水平相关,肿瘤细胞表面MICA/B的表达水平影响NK细胞的杀伤活性。Objective To analyze the relationship between the expression of MHC class I chain-related gene A/B （MICA/B） on different human cancer cells and their sensitivity to NK cell cytotoxicity. Methods The expression MICA/B in K562, Bcap37,769P and A549 cells was measured by FACS. Isolation of NK cells were obtained by the modified RosetteSep procedure. Cytotoxicity of NK cells at different effeet-te-target ratios （E：T）were determined by MTT assay. Results The expression of MICA/MICB on 4 tumor cell lines were （60.35±0.50）%, （90.72±0.64）%, （55.59±0.55）%,and （3.84±0.10）% respectively. The purity of NK cells obtained by the modified RosetteSep procedure was （96.52±2.42）%,whereas the cytotoxicity of NK cells against K562,Bcap39 and 769P was much higher than that of A549（P 〈 0.01 ）. The expression of MICA/B was significantly correlated with the cytotoxicity of NK cells at E：T ratios of 5：1,10：1 and 20：1 respectively （P 〈0.01 ）. Conclusion The MICA/B expression on cancer cell lines was correlated with NK celt-mediated cytolysis and influenced the cytotoxicity of NK cells.

关 键 词：自然杀伤细胞 肿瘤细胞 MICA/B 细胞毒性实验 

分 类 号：R730.3[医药卫生—肿瘤]