阿霉素诱导食管癌耐药细胞中ABCG2的表达及其意义  

Expression of ABCG2 in Adriamycin-resistant Human Esophageal Cancer Cells

作　　者：刘亮[1] 左静[2] 赵丽[1] 王静[1] 郭建文[1] 刘江惠[1] 左连富[1] 

机构地区：[1]河北医科大学第四医院肿瘤研究所流式细胞室,石家庄050011 [2]河北医科大学第四医院肿瘤内科,石家庄050011

出　　处：《中国医科大学学报》2010年第2期101-104,共4页Journal of China Medical University

基　　金：河北省普通高等学校强势特色学科-肿瘤学建设经费资助项目(冀教高[2005]52号);河北省自然科学基金资助项目(C2007001060)

摘　　要：目的研究阿霉素(ADM)药物诱导食管癌细胞(Eca109)产生耐药细胞株(Eca109/ADM)中ATP结合转运蛋白G超家族成员2(ABCG2)的表达及其意义。方法通过逐步增加培养液中阿霉素的浓度,长期筛选培养,建立食管癌耐药细胞株Eca109/ADM。逆转录聚合酶链反应(RT-PCR)方法检测耐药细胞中ABCG2mRNA的表达量,流式细胞术(FCM)和蛋白印迹(Westernblot)方法检测耐药细胞中ABCG2蛋白的表达量及细胞定位。FCM方法检测Eca109/ADM细胞药物外排作用,MTT方法检测Eca109/ADM细胞对阿霉素的耐药系数。结果Eca109/ADM细胞与Eca109细胞相比,ABCG2表达显著性增高(P<0.05)。Eca109/ADM细胞药物外排作用较Eca109细胞增加,Eca109/ADM细胞对阿霉素的耐药系数为3.29。结论成功建立耐药细胞株Eca109/ADM。Eca109/ADM细胞是一个理想的耐药细胞模型。Eca109/ADM细胞中ABCG2的表达量增高,提示ABCG2参与食管癌细胞耐药的形成。Objective To explore the expression of ATP-binding cassette transporter G2 （ABCG2） in adriamycin （ADM）-resistant human esophageal cancer cells. Melhods The ADM-resistant human esophageal cancer cell （Ecal09/ADM） was induced by gradually increasing the ADM concentration in the culture medium of human esophageal cancer cell line（Ecal09） and long time screening culture. ABCG2 mRNA and protein of ADM-resistant cells was detected by RT-PCR,flow cytometry （FCM） and Westem blot. Drug excretion of Ecal09/ADM cells was examined by FCM. The drug resistance index to ADM was detected by MTT. Results The expression of ABCG2 in Eca109/ADM cells was higher than that in Ecal09 cells. The drug excretion of Ecal09/ADM cells was stronger than Eca109 cells. The Eca109/ADM cells drug resistance index to ADM was 3.29. Conclusion The ADM-resistant cell line Eca109/ADM was established successfully as an ideal chemoresistant cell model. ABCG2 might be involved in the drug resistance of esophageal cancer cell.

关 键 词：ATP结合转运蛋白G超家族成员2 流式细胞术 阿霉素 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]