菌种L3深层发酵产漆酶及其对废水脱色的研究  

Laccase produced by using submerged fermentation of Trametes versicolor SYBC-L3 and its effect on the decolorization of wastewater

作　　者：刘家扬[1] 蔡宇杰[1] 廖祥儒[1] 王欣[1] 张峰[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《工业水处理》2010年第2期27-30,共4页Industrial Water Treatment

基　　金：长江学者和创新团队发展计划资助(IRT0532)

摘　　要：对菌种Trametes versicolor SYBC-L3发酵产漆酶的碳氮源进行了优化,研究了菌株L3漆酶的性质及其在毛纺废水脱色中的作用。结果表明,其最佳发酵碳氮源为:麦芽糖12g/L、豆粕4g/L,优化后的漆酶的酶活在第8天可高达48400U/L;酶最适反应温度为50℃,在70℃以下保温1h,仍然有70%的剩余活力,其最适pH为3.5,在pH4～8下保温24h,仍然有70%以上的活力。用L3漆酶处理毛纺废水,20min脱色率达到70%,有较好的脱色效果。Optimization of medium components for the production of laccase by the strain Trametes versicolor. SYBC-L3 has been studied and the effect of the laccase on woolen textile decolorization has been detected with the collected wastewater from a woolen textile factory. Its optimal carbon and nitrogen sources are as follows ： maltose 12 g/L, soybean meal 4g/L. The optimized laccase activity reaches 48 400 U/L on the 8th day which was significantly higher than that was previously reported. The characteristics of laccase are also investigated. It shows that the most suitable reaction temperature is 50℃. When the temperature keeps at 70℃ for 1 h, the remained activity is still 70%. Its most suitable pH is 3.5. When pH is 4-8, and the temperature keeps for 24 h, the remained activity is still 70%. When the woolen textile wastewater is treated with laccase L3 for 20 min, 70% of decolorization rate has been achieved, indicating the decolorization capacity of laccase is quite good.

关 键 词：白腐真菌 漆酶 脱色 

分 类 号：Q939.9[生物学—微生物学] X791[环境科学与工程—环境工程]