多种化疗药物对胃癌细胞株MKN-45细胞周期动力学的影响  

Effects of chemotherapeutic agents on the cell cycle of line of gastric carcinoma MKN-45 cell line

作　　者：欧阳春[1] 莫剑忠[1] 童菊芳[1] 曾民德[1] 萧树东[1] 江绍基[1] 石学耕[2] 

机构地区：[1]上海市消化疾病研究所,200001 [2]上海第二医科大学生物物理教研室

出　　处：《中华消化杂志》1998年第6期328-330,共3页Chinese Journal of Digestion

摘　　要：目的体外研究化学药物对胃癌细胞株 MKN-45的细胞周期影响。方法应用Brdu/DNA 双标记 FCM 技术,观察多种化疗药物对 MKN-45细胞周期动力学的影响。结果 5-Fu、VP16和 MMC 能显著影响肿瘤细胞的周期动力学,阻抑肿瘤细胞生长;甲酰四氢叶酸能加强5-Fu 的作用。而加用阿霉素、顺氨氯铂并不加强5-Fu 或 MMC 的作用。结论研究了化疗药物对胃癌细胞株的细胞周期动力学改变,为临床化疗药物应用提供参考数据。Objective We investigate the effects of chemotherapeutic agents on the cell cycle of gastric carcinoma,MKN-45 cell line,in vitro.Methods The effects of several chemotherapeutic agents on cell cycle kinetics of MKN-45 were observed by means of Brdu/DNA double-labelling technique.Results 5-Fu,VP16, and MMC influenced significantly the cell cycle kinetics of tumor cell,and inhibit the cell growth;leucovorin increased the effect of 5-Fu,whereas doxorubicin, cisplatin did not increase the effect of 5-Fu or MMC.Conclusion The present study provides references data for clinical use of chemotherapeutic agents.

关 键 词：胃癌细胞 细胞周期动力学 药物疗法 胃癌 

分 类 号：R735.205[医药卫生—肿瘤]