皱纹盘鲍淀粉酶和褐藻酸酶的研究  

THE PROPERTIES OF AMYLASE AND ALGALASE IN HALIOTIS DISCUS HANNAI

作　　者：杨蕙萍[1,2] 童圣英[1,2] 王子臣[1,2] 

机构地区：[1]中国科学院海洋研究所 [2]大连水产学院养殖系

出　　处：《水产学报》1998年第4期345-351,共7页Journal of Fisheries of China

摘　　要：利用分光光度计比色法测定了２～３ｃｍ的皱纹盘鲍（ＨａｌｉｏｔｉｓｄｉｓｃｕｓｈａｎｎａｉＩｎｏ）的淀粉酶和褐藻酸酶的最适温度和最适ｐＨ以及１１种金属离子对其的影响。结果表明：最适温度分别为３０℃、３５℃，最适ｐＨ分别为６．２４、７．１９，在最适温度下的活化能分别为５．０３×１０４Ｊ／ｍｏｌ和１．３３×１０４Ｊ／ｍｏｌ；皱纹盘鲍的三种主要消化酶的活性依次为：褐藻酸酶＞蛋白酶＞淀粉酶。Ｃｕ２＋、Ｈｇ２＋、Ａｇ＋、Ｐｂ２＋对淀粉酶活性具有显著的抑制作用，其他离子则具有促进作用，其中又以Ｍｎ２＋、Ｂａ２＋、Ｃａ２＋三种金属离子尤为突出，其促进作用比对照组高一倍以上。而对褐藻酸酶，Ｚｎ２＋、Ａｇ＋、Ｈｇ２＋、Ｌｉ２＋、Ｂａ２＋具显著的抑制作用，其他离子则没有作用。The amylase and algalase of the Pacific abalone(Haliotis discus hannai) were measured during the length of 2-3 cm by means of spectrophotometer. The optimum temperatures for amylase and algalase were 30℃ and 35℃, The optimum pH values were 6.24 and 7.19. Under the optimum temperature the active energy of two enzymes were 5.03×10 4 J/mol and 1.33×10 4 J/mol respectively; the activity of amylase and algalase were detected when eleven metal ions existed. The results indicated that Cu 2+ 、Hg 2+ 、Ag +、Pb 2+ significantly inhibited the activity of amylase, and other metal ions activated the activity of amylase. Mn 2+ , Ba 2+ and Ca 2+ can activate the amylase two times of that in control group. For the algalase, Zn 2+ , Ag +, Hg 2+ , Li 2+ and Ba 2+ significantly inhibited its activity, and the other metal ions had no effects on the algalase.

关 键 词：皱纹盘鲍 淀粉酶 褐藻酸酶 金属离子 

分 类 号：Q959.212[生物学—动物学]