钙离子载体及咖啡因对绵羊精子获能的影响  

Effects of Calcium Ionophore and Caffeine on Ovine Sperm Capacitation in vitro

作　　者：刘烈琴[1] 郭勇[1] 刘云海[1] 薛君忠[1] 张宏[1] 李晓燕[1] 倪和民[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国畜牧兽医》2010年第1期112-116,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金面上项目(30871836);十一五国家支撑计划二级子项目(2008BADB2B04-4)

摘　　要：通过添加不同浓度（0.05、0.1、0.2、0.5、1、5、10μmol/L）的钙离子载体（A23187,IA）及2 mmol/L咖啡因,探讨不同作用时间其对绵羊精子体外获能的影响。结果发现,IA浓度越高,精子的体外获能率越高,顶体反应率越低,活力越低,死精子就越多;而延长作用时间对获能、顶体反应的影响不明显。IA和咖啡因共同作用后,咖啡因能够促进IA诱导顶体反应的发生。建议用IA诱导绵羊精子获能时,其浓度以0.050.2μmol/L为宜,作用时间1 min。It was aimed to investigate the effects of different concentrations（0.05,0.1,0.2,0.5,1,5,10 μmol/L） of calcium ionophore（A23187,IA） and 2 mmol/L caffeine on the ovine sperm capacitation in vitro.The results showed that the higher rate of capacitation in vitro,lower the rate of acrosome reaction（AR）,and the lower sperm motilily were induced by the high concentration of IA,but the dead sperm could be increased.The capacitation and AR of ovine sperm in vitro were not affected even delaying time.Caffeine could promote IA to induce AR.Collectively,this might be recommol/Lended that it be applicable for improving ovine capacitation in vitro with 0.05 to 0.2 μmol/L IA for 1 min.

关 键 词：绵羊精子 体外获能 钙离子载体 咖啡因 

分 类 号：S814[农业科学—畜牧学]