机构地区:[1]江苏省无锡市疾病预防控制中心,214023 [2]南京医科大学公共卫生学院流行病与卫生统计学系
出 处:《中华预防医学杂志》2010年第3期242-246,共5页Chinese Journal of Preventive Medicine
基 金:无锡市卫生系统指令性项目(XM0709)
摘 要:目的探讨中国女性人群DNA修复基因X线修复交叉互补基因1(XRCCl)Argl94Trp、Arg399Gln多态与乳腺癌易感性的关系。方法采用病例对照研究设计,包括经组织病理学确诊的女性乳腺癌患者698例和按地区、年龄频数匹配的对照人群813名,以聚合酶链反应-限制性片段长度多态性(PCR—RFLP)方法进行多态性检测,应用logistic回归计算OR值及95%CI值,运用Meta分析估计Arg399Gln与中国女性人群乳腺癌危险性的关联。结果XRCCI基因194位点3种基因型Arg/Arg、A形Trp、Trp/Trp,及Arg/Trp+Trp/Trp在病例组分布频率分别为48.81%(327/670)、39.85%(267/670)、11.34%(76/670)和51.19%(343/670),在对照组中分别为48.80%(387/793)、41.99%(333/793)、9.21%(73/793)和51.20%(406/793)。与Arg/Arg比较各基因型的校正OR值(95%CI值)分别为0.98(0.75~1.28)、1.17(0.76~1.80)、1.09(0.86~1.40)。399位点Arg/Arg、Arg/Gin、Gin/Gin和Arg/Gin+Gin/Gin基因型频率在病例组分别为52.40%(349/666)、38.29%(255/666)、9.31%(62/666)和47.60%(317/666),对照组分别为52.22%(412/789)、38.53%(304/789)、9.25%(73/789)和47.78%(377/789)。与Arg/Arg比较各基因型的校正OR值(95%CI值)分别为0,93(0.63~1.08)、0.96(0.42~1.09)、0.91(0.62~1.05)。未见两位点多态与乳腺癌危险性之间存在关联。分别以绝经状况、哺乳、生育、口服避孕药史进行分层,未发现两多态位点与乳腺癌危险性的显著关联。Meta分析结果提示Arg399Gln位点与中国女性乳腺癌无相关性(OR=0.97,95%CI:0.85~1.10)。结论XRCCl基因Argl94Trp和Arg399Gln多态性可能不是中国女性人群乳腺癌的易感性标志物。Objective To study the relationship between two polymorphisms, Arg194Trp and Arg399Glu,of DNA repair gene X-ray repair cross-complementing group 1 (XRCCl) and the susceptibility of breast cancer in Chinese women. Methods A case-control study with 698 histologically-confirmed female breast cancer cases and 813 cancer-free controls frequency-matched by age and residential area was conducted ,and the genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays. Logistic regression analysis was used to evaluate the odds ratios (ORs) and 95% confidence intervals (Cls) of XRCCl Arg194Trp and Arg399Glu with susceptibility of breast cancer. A Meta-analysis was used to evaluate the association of Arg399Glu with breast cancer in Chinese women. Results The genotype frequencies of Arg/Arg, Arg/Trp, Trp/Trp, Arg/Trp + Trp/Trp of XRCCl gene 194 locus were 48.81% ( 327/670 ), 39.85 % ( 267/670 ), 11.34% ( 76/670 ), 51.19% ( 343/670 ) in cases and 48. 80% (387/793), 4l. 99% (333/793), 9.21% (73/793), 51.20% (406/793) in controls. Compared to Arg/Arg, the adjusted ORs ( 95% Cls ) were 0.98 ( 0.75 - 1.28 ), 1.17 ( 0. 76 -1.80), 1.09 ( 0.86 - 1.40). The frequencies of Arg/Arg, Arg/Trp, Trp/Trp, Arg/Gln + Gin/Gin of XRCCl 399 locus were 52.40% ( 349/666 ), 38.29% ( 255/666 ), 9.31% ( 62/666 ), 47.60% ( 317/666 ) in cases and 52. 22% (412/789) ,38.53% (304/789) ,9. 25% (73/789) ,47.78% (377/789)in controls. Compared to Arg/Arg, the adjusted ORs ( 95% Cls) were 0.93 ( 0. 63 - 1.0B ), 0.96 ( 0. 42 - 1.09 ), 0.91 ( 0.62 - 1.05). No significant associations were found between these two polymovphisms and breast cancer risk, also in subgroups stratified by menopause status, history of breast-feed, reproduction and taking oral contraceptives. The overall ORs (95% Cls) of 399 Arg/Trp + Trp/Trp vs Arg/Arg from Meta analysis was 0. 97 (0. 85 - 1. 10). Conclusion T
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